Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Mouse monoclonal [JJ6] to p23
Suitable for: WB, ICC/IF, IHC
Reacts with: Mouse, Human
Isotype: IgG1

Product name

Anti-p23 antibody [JJ6]
See all p23 primary antibodies
Description

Mouse monoclonal [JJ6] to p23
Host species

Mouse

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC	Mouse
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein corresponding to Human p23.
Database link: Q15185
Positive control
- ICC/IF: HaCaT cells and heat shocked HeLa cells. IHC: Mouse back skin. WB: Mixture of human cell lines, cell lysate.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein G purified
Clonality

Monoclonal
Clone number

JJ6
Isotype

IgG1
Research areas

Western blot - Anti-p23 antibody [JJ6] (ab223608)

Anti-p23 antibody [JJ6] (ab223608) at 1/1000 dilution + Mixture of human cell lines, cell lysate at 15 µg

Secondary
Sheep Anti-Mouse IgG: HRP

Predicted band size: 19 kDa

Blocking: 1.5% BSA for 30 minutes at RT.

The cell line mixture contains: A431 (human epidermoid carcinoma cell line), A549 (human lung carcinoma cell line), HCT116 (human colon cancer), HeLa (human epithelial cell line from cervix adenocarcinoma), HEK-293 (human epithelial cell line from embryonic kidney), HepG2 (human liver hepatocellular carcinoma cell line), HL-60 (human promyelocytic leukemia cell line), HUVEC (human umbilical vein endothelial cell line), Jurkat (human T cell leukemia cell line from peripheral blood), MCF7 (human breast adenocarcinoma cell line), PC-3 (human prostate adenocarcinoma cell line) and T98G (human brain glioblastoma).
Immunohistochemistry - Anti-p23 antibody [JJ6] (ab223608)

Bouin's fixed-paraffin embedded mouse back skin tissue stained for p23 with ab223608 (green) (1/100) in immunohistochemical analysis. Secondary antibody: FITC Goat Anti-Mouse at 1/50.
Immunocytochemistry/ Immunofluorescence - Anti-p23 antibody [JJ6] (ab223608)

2% formaldehyde fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for p23 (green) using ab223608 (1/100) in ICC/IF. Secondary Antibody: FITC Goat Anti-Mouse at 1/200. DAPI counterstain (blue).
Immunocytochemistry/ Immunofluorescence - Anti-p23 antibody [JJ6] (ab223608)

100% methanol fixed HaCaT (human keratinocyte cell line) cells stained for p23 (green) using ab223608 (1/100) in ICC/IF. Secondary Antibody: FITC Goat Anti-Mouse at 1/50.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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