Human Apo-H ELISA Kit (Apolipoprotein H) (ab274403)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 10.4 pg/ml
- Range: 23.44 pg/ml - 1500 pg/ml
- Sample type: Cell Lysate, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Apo-H ELISA Kit (Apolipoprotein H)
See all Apo-H kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 1.7% Inter-assay Sample n Mean SD CV% Serum 3 2.99% -
Sample type
Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
10.4 pg/ml -
Range
23.44 pg/ml - 1500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 100 95% - 104% Cell Lysate 93 88% - 101% Hep Plasma 103 94% - 118% EDTA Plasma 101 96% - 107% Cit plasma 99 96% - 107% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Apo-H ELISA kit (ab27403) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Apo-H protein in serum, plasma and lystates. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Apo-H with 10.4 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Apo-H, also known as beta-2 glycoprotein 1, is a key serum protein involved in agglutination response in the blood. It contains a highly positively charged stretch of amino acids which bind negatively charged agglutinizing factors. Improper expression of apo-H has been noted in many vascular disease states such as, cardiovascular disease, diabetes, and thrombosis. The immunogen used to raise the antibodies for this kit was the full-length mature protein. This protein does not share high sequence identity in mouse or rat samples (76.1 and 82.2%, respectively) and, therefore, is not expected to cross-react.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Apo-H (Apolipoprotein H) Capture Antibody 1 x 600µl 10X Human Apo-H (Apolipoprotein H) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4B 1 x 6ml Human Apo-H (Apolipoprotein H) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Sequence similarities
Contains 4 Sushi (CCP/SCR) domains. -
Post-translational
modificationsN- and O-glycosylated. PubMed:6587378 also reports glycosylation on 'Asn-188' for their allele. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Activated protein C binding protein
- Activated protein C-binding protein
- Anticardiolipin cofactor
see all -
Database links
- Entrez Gene: 350 Human
- Omim: 138700 Human
- SwissProt: P02749 Human
- Unigene: 445358 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Apo-H standard curve in Sample Diluent NS.The Apo-H standard curve was prepared as described in Section 10.1. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Apo-H in human serum and plasma samples.The concentrations of Apo-H were measured in duplicates, interpolated from the Apo-H standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:140,000, plasma (Heparin) 1:80,000, plasma (EDTA) 1:80,000 and plasma (Citrate) 1:80,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apo-H concentration was determined to be 164.87 mg/mL in serum, 91.63 mg/mL in plasma (Heparin) and 82.17 mg/mL in plasma (EDTA) and 82.25 mg/mL in plasma (Citrate).
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Interpolated concentrations of native Apo-H in human HepG2 Cell extract based on a 200 µg/mL extract load.The concentrations of Apo-H were measured in duplicate and interpolated from the Apo-H standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apo-H concentration was determined to be 1307.93 pg/mL.
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Sandwich ELISA - Human Apo-H ELISA Kit (Apolipoprotein H) (ab274403)To learn more about the advantages of recombinant antibodies see here.
