Human Apo-H Antibody Pair - BSA and Azide free (Apolipoprotein H) (ab253425)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human
Overview
-
Product name
Human Apo-H Antibody Pair - BSA and Azide free (Apolipoprotein H)
See all Apo-H kits -
Assay type
ELISA set -
Range
23.438 pg/ml - 1500 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
The Antibody Pair can be used to quantify Human Apo-H. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab274403), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
-
Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Human Apo-H (Apolipoprotein H) Capture Antibody (unconjugated) 1 x 100µg Human Apo-H (Apolipoprotein H) Detector Antibody (unconjugated) 1 x 100µg -
Research areas
-
Function
Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Sequence similarities
Contains 4 Sushi (CCP/SCR) domains. -
Post-translational
modificationsN- and O-glycosylated. PubMed:6587378 also reports glycosylation on 'Asn-188' for their allele. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- Activated protein C binding protein
- Activated protein C-binding protein
- Anticardiolipin cofactor
see all -
Database links
- Entrez Gene: 350 Human
- Omim: 138700 Human
- SwissProt: P02749 Human
- Unigene: 445358 Human
Images
-
Sandwich ELISA - Human Apo-H Antibody Pair - BSA and Azide free (Apolipoprotein H) (ab253425)To learn more about the advantages of recombinant antibodies see here.
