Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human Factor X ELISA Kit (ab108832)

Key features and details

  • Sensitivity: 1.5 ng/ml
  • Range: 0.781 ng/ml - 50 ng/ml
  • Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human Factor X ELISA Kit
    See all Factor X kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 20 = 3.2%
    2 20 = 3.4%
    3 20 = 3.7%
    Inter-assay
    Sample n Mean SD CV%
    1 20 = 6.2%
    2 20 = 6.8%
    3 20 = 7.2%

  • Sample type

    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    = 1.5 ng/ml

  • Range

    0.781 ng/ml - 50 ng/ml

  • Recovery

    97.5 %

  • Assay time

    4h 00m

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Abcam’s Human Factor Xin vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor X concentrations in plasma, serum, milk, urine, saliva, cerebrospinal fluid and cell culture supernatants.


    A Factor X specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor X specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor X captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Antibody 1 x 120µl
    Chromogen Substrate 1 x 7ml
    Factor X Microplate (12 x 8 well strips) 1 unit
    Factor X Standard 1 x 65ng
    Sealing Tapes 3 units
    Stop Solution 1 x 11ml

  • Research areas

  • Function

    Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.

  • Tissue specificity

    Plasma; synthesized in the liver.

  • Involvement in disease

    Defects in F10 are the cause of factor X deficiency (FA10D) [MIM:227600]. A hemorrhagic disease with variable presentation. Affected individuals can manifest prolonged nasal and mucosal hemorrhage, menorrhagia, hematuria, and occasionally hemarthrosis. Some patients do not have clinical bleeding diathesis.

  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 2 EGF-like domains.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 1 peptidase S1 domain.

  • Post-translational
    modifications

    The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
    N- and O-glycosylated.
    The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • Activated factor Xa heavy chain
    • Coagulation factor X
    • F10
    • FA10_HUMAN
    • factor Xa
    • FX
    • FXA
    • Prothrombinase
    • Stuart factor
    • Stuart Prower factor
    • Stuart-Prower factor
    see all

  • Database links

    Images

    • ELISA: Factor X Human ELISA kit (ab108832)
      ELISA: Factor X Human ELISA kit (ab108832)

      Standard curve with background signal subtracted (duplicates; +/- SD).

    • ELISA: Factor X Human ELISA kit (ab108832)
      ELISA: Factor X Human ELISA kit (ab108832)

      Factor X measured in undiluted culture supernatants with MCF7 levels below level of detection (duplicates +/- SD).

    • ELISA: Factor X Human ELISA kit (ab108832)
      ELISA: Factor X Human ELISA kit (ab108832)

      Factor X measured in biological fluids (duplicates +/- SD). Serum and plasma were tested at 1/300-1/3000; other samples at 1/1-1/30.

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab108832

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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