Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cyclophilin B antibody [EPR12703(B)] - BSA and Azide free (ab238991)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12703(B)] to Cyclophilin B - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Cyclophilin B antibody [EPR12703(B)] - BSA and Azide free
    See all Cyclophilin B primary antibodies

  • Description

    Rabbit monoclonal [EPR12703(B)] to Cyclophilin B - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2, HeLa, NIH/3T3, PC-12, HAP1, Jurkat, U87-MG and A431 cell lysates, mouse and rat heart tissue lysates.

  • General notes

    Ab238991 is the carrier-free version of ab178397. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238991 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Cyclophilin B antibody [EPR12703(B)] - BSA and Azide free (ab238991)
    Western blot - Anti-Cyclophilin B antibody [EPR12703(B)] - BSA and Azide free (ab238991)
    All lanes : Anti-Cyclophilin B antibody [EPR12703(B)] - Loading Control (ab178397) at 1/1000 dilution

    Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : PPIB knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
    Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 24 kDa
    Observed band size: 24 kDa



    This data was developed using ab178397, the same antibody clone in a different buffer formulation.

    Lanes 1-4: Merged signal (red and green). Green - ab178397 observed at 24 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab178397 Anti-Cyclophilin B antibody [EPR12703(B)] was shown to specifically react with Cyclophilin B in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261746 (knockout cell lysate ab257037) was used. Wild-type and Cyclophilin B knockout samples were subjected to SDS-PAGE. ab178397 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Cyclophilin B antibody [EPR12703(B)] - BSA and Azide free (ab238991)
    Western blot - Anti-Cyclophilin B antibody [EPR12703(B)] - BSA and Azide free (ab238991)
    All lanes : Anti-Cyclophilin B antibody [EPR12703(B)] - Loading Control (ab178397) at 1 µg/ml

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : PPIB knockout HAP1 whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : U87-MG whole cell lysate

    Lysates/proteins at 20 µg/ml per lane.

    Predicted band size: 24 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178397).

    Lanes 1 - 4: Merged signal (red and green). Green - ab178397 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab178397 was shown to recognize PPIB in wild-type HAP1 cells as signal was lost at the expected MW in PPIB knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PPIB knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab178397 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Cyclophilin B antibody [EPR12703(B)] - BSA and Azide free (ab238991)
    Western blot - Anti-Cyclophilin B antibody [EPR12703(B)] - BSA and Azide free (ab238991)
    All lanes : Anti-Cyclophilin B antibody [EPR12703(B)] - Loading Control (ab178397) at 1/1000 dilution

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 3 : A431 (Human epidermoid carcinoma cell line) cell lysate

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 24 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab178397).

  • Anti-Cyclophilin B antibody [EPR12703(B)] - BSA and Azide free (ab238991)
    Anti-Cyclophilin B antibody [EPR12703(B)] - BSA and Azide free (ab238991)

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