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Mouse FX ELISA kit (total FX antigen) (ab272779)

Key features and details

  • Range: 2.5 ng/ml - 500 ng/ml
  • Sample type: Cit plasma, Other biological fluids
  • Detection method: Colorimetric
  • Assay type: Quantitative
  • Reacts with: Mouse

Overview

  • Product name

    Mouse FX ELISA kit (total FX antigen)
    See all Factor X kits

  • Detection method

    Colorimetric

  • Sample type

    Other biological fluids, Cit plasma

  • Assay type

    Quantitative

  • Range

    2.5 ng/ml - 500 ng/ml

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    Mouse FX ELISA kit (total FX antigen) (ab272779) is for the quantitative determination of total Factor X and Xa in biological fluids.


    Mouse Factor X will bind to the affinity purified capture antibody coated on the microtiter plate. Factor X, Xa, and Xa in complex with inhibitors will react with the antibody on the plate. After appropriate washing steps, biotin labeled polyclonal anti-mouse Factor X primary antibody binds to the Factor X. Excess antibody is washed away and bound polyclonal antibody is then reacted with Streptavidin conjugated to horseradish peroxidase. Following an additional washing step, TMB substrate is used for color development at 450nm. The amount of color development is directly proportional to the concentration of total Factor X in the sample.

  • Tested applications

    Suitable for: ELISAmore details

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer 1 x 50ml
    Anti-Mouse FX Primary Antibody Lyophilized Vial 1 vial
    Mouse FX ELISA Plate 1 unit
    Mouse FX Standard Lyophilized Vial 1 vial
    Streptavidin-HRP Secondary Reagent 1 vial
    TMB Substrate 1 x 10ml

  • Research areas

  • Function

    Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.

  • Tissue specificity

    Plasma; synthesized in the liver.

  • Involvement in disease

    Defects in F10 are the cause of factor X deficiency (FA10D) [MIM:227600]. A hemorrhagic disease with variable presentation. Affected individuals can manifest prolonged nasal and mucosal hemorrhage, menorrhagia, hematuria, and occasionally hemarthrosis. Some patients do not have clinical bleeding diathesis.

  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 2 EGF-like domains.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 1 peptidase S1 domain.

  • Post-translational
    modifications

    The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
    N- and O-glycosylated.
    The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • Activated factor Xa heavy chain
    • Coagulation factor X
    • F10
    • FA10_HUMAN
    • factor Xa
    • FX
    • FXA
    • Prothrombinase
    • Stuart factor
    • Stuart Prower factor
    • Stuart-Prower factor
    see all

  • Database links

    Images

    • Example data
      Example data

      A typical standard curve. Example only.

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