Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Factor VIII antibody [EPR24039-262] - BSA and Azide free (ab275383)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24039-262] to Factor VIII - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Factor VIII antibody [EPR24039-262] - BSA and Azide free
    See all Factor VIII primary antibodies

  • Description

    Rabbit monoclonal [EPR24039-262] to Factor VIII - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC, Flow Cytmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human plasma; HUVEC whole cell lysate. IHC-P: Human colon carcinoma and bladder cancer tissue; Mouse spleen and lung tissue; Rat lung tissue. ICC: HUVEC cells. Flow cyt: HUVEC cells.

  • General notes

    ab275383 is the carrier-free version of ab275376. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab275383 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR24039-262

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VIII antibody [EPR24039-262] - BSA and Azide free (ab275383)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VIII antibody [EPR24039-262] - BSA and Azide free (ab275383)

    This data was developed using ab275376, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling Factor VIII with ab275376 at 1/1000 (0.457 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the blood vessels in human colon carcinoma (PMID: 18846440). The section was incubated with ab275376 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Flow Cytometry - Anti-Factor VIII antibody [EPR24039-262] - BSA and Azide free (ab275383)
    Flow Cytometry - Anti-Factor VIII antibody [EPR24039-262] - BSA and Azide free (ab275383)

    This data was developed using ab275376, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HEK-293 (Human embryonic kidney epithelial cell, Left) / HUVEC (Human umbilical vein endothelial cell, Right) cells labelling Factor VIII with ab275376 at 1/500 dilution. 

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Negative control: HEK-293 (PMID: 31727959). HUVEC cells express Factor VIII is reported in literature (PMID: 30814851).

  • Immunocytochemistry - Anti-Factor VIII antibody [EPR24039-262] - BSA and Azide free (ab275383)
    Immunocytochemistry - Anti-Factor VIII antibody [EPR24039-262] - BSA and Azide free (ab275383)

    This data was developed using ab275376, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 100% methanol-fixed HUVEC cells labelling Factor VIII with ab275376 at 1/1000 (0.457 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HUVEC cell line. Negative control: HEK-293 (PMID: 31727959). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Western blot - Anti-Factor VIII antibody [EPR24039-262] - BSA and Azide free (ab275383)
    Western blot - Anti-Factor VIII antibody [EPR24039-262] - BSA and Azide free (ab275383)
    Anti-Factor VIII antibody [EPR24039-262] (ab275376) at 1/1000 dilution + Human plasma at 20 µl

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 267 kDa
    Observed band size: 210-280,330 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab275376, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 3085106).

    Exposure time: 10 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VIII antibody [EPR24039-262] - BSA and Azide free (ab275383)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VIII antibody [EPR24039-262] - BSA and Azide free (ab275383)

    This data was developed using ab275376, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling Factor VIII with ab275376 at 1/1000 (0.457 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on rat lung (PMID: 25911555). The section was incubated with ab275376 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-Factor VIII antibody [EPR24039-262] - BSA and Azide free (ab275383)
    Western blot - Anti-Factor VIII antibody [EPR24039-262] - BSA and Azide free (ab275383)
    All lanes : Anti-Factor VIII antibody [EPR24039-262] (ab275376) at 1/1000 dilution

    Lane 1 : HUVEC (human umbilical vein endothelial cell), whole cell lysate
    Lane 2 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 267 kDa
    Observed band size: 166,210-280,330 kDa why is the actual band size different from the predicted?



    This data was developed using ab275376, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: HEK-293 (PMID: 27208572).

    The molecular weight observed is consistent with what has been described in the literature (PMID: 3085106).

    Exposure time: 26 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VIII antibody [EPR24039-262] - BSA and Azide free (ab275383)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VIII antibody [EPR24039-262] - BSA and Azide free (ab275383)

    This data was developed using ab275376, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Factor VIII with ab275376 at 1/1000 (0.457 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on mouse lung (PMID: 25911555). The section was incubated with ab275376 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VIII antibody [EPR24039-262] - BSA and Azide free (ab275383)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VIII antibody [EPR24039-262] - BSA and Azide free (ab275383)

    This data was developed using ab275376, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Factor VIII with ab275376 at 1/1000 (0.457 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the megakaryocytes and platelets in mouse spleen (PMID: 25911555). The section was incubated with ab275376 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VIII antibody [EPR24039-262] - BSA and Azide free (ab275383)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VIII antibody [EPR24039-262] - BSA and Azide free (ab275383)

    This data was developed using ab275376, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissue labeling Factor VIII with ab275376 at 1/1000 (0.457 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the blood vessels in human bladder cancer. The section was incubated with ab275376 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-Factor VIII antibody [EPR24039-262] - BSA and Azide free (ab275383)
    Anti-Factor VIII antibody [EPR24039-262] - BSA and Azide free (ab275383)

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