Anti-Factor VIII antibody [GMA-012] (ab78852)
![Anti-Factor VIII antibody [GMA-012] (ab78852)](https://www.abcam.com/ps/products/78/ab78852/Images/Factor-VIII-Primary-antibodies-ab78852-16.jpg "Anti-Factor VIII antibody [GMA-012] (ab78852)")
Key features and details
- Mouse monoclonal [GMA-012] to Factor VIII
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Factor VIII antibody [GMA-012]
See all Factor VIII primary antibodies -
Description
Mouse monoclonal [GMA-012] to Factor VIII -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Purified human Factor VIII
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Positive control
- Human Factor VIII. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal liver.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituents: 1% Mannitol, 0.87% Sodium chloride, 0.164% Sodium phosphate -
Concentration information loading... -
Purity
DEAE-Chromatography -
Clonality
Monoclonal -
Clone number
GMA-012 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Factor VIII antibody [GMA-012] (ab78852)Anti-Factor VIII antibody [GMA-012] (ab78852) at 1 µg/ml + human Factor VIII at 2 µg
Secondary
Goat anti-mouse HRP conjugated IgG
Predicted band size: 267 kDa
Observed band size: 280 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VIII antibody [GMA-012] (ab78852)](https://www.abcam.com/ps/products/78/ab78852/Images/Factor-VIII-Primary-antibodies-ab78852-17.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VIII antibody [GMA-012] (ab78852)IHC image of Factor VIII staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78852, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
