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Cancer Tumor biomarkers Enzymes Phosphatases

Anti-SHP1 antibody (ab227503)

Price and availability

278 083 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-SHP1 antibody (ab227503)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to SHP1
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-SHP1 antibody
    See all SHP1 primary antibodies
  • Description

    Rabbit polyclonal to SHP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment within Human SHP1 (internal sequence). The exact sequence is proprietary.
    Database link: P29350

  • Positive control

    • WB: Rat liver extract; HeLa S3, HepG2 and NIH/3T3 whole cell lysates. IHC-P: OVCA xenograft tissue. IP: HeLa whole cell lysate (ab150035).
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Phosphatases
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Cancer
    • Cell cycle
    • Kinases/phosphatases
    • Phosphatases
    • Cancer
    • Signal transduction
    • G protein signaling
    • GPCR
    • Developmental Biology
    • Organogenesis
    • Hematopoietic system development

Images

  • Western blot - Anti-SHP1 antibody (ab227503)
    Western blot - Anti-SHP1 antibody (ab227503)
    Anti-SHP1 antibody (ab227503) at 1/1000 dilution + Rat liver extract at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 68 kDa



    7.5% SDS-PAGE gel.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody (ab227503)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody (ab227503)

    Paraffin-embedded OVCA (human) xenograft tissue stained for SHP1 using ab227503 at 1/100 dilution in immunohistochemical analysis.

  • Western blot - Anti-SHP1 antibody (ab227503)
    Western blot - Anti-SHP1 antibody (ab227503)
    All lanes : Anti-SHP1 antibody (ab227503) at 1/500 dilution

    Lane 1 : HeLa S3 whole cell lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 68 kDa



    7.5% SDS-PAGE gel.

  • Western blot - Anti-SHP1 antibody (ab227503)
    Western blot - Anti-SHP1 antibody (ab227503)
    Anti-SHP1 antibody (ab227503) at 1/1000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 68 kDa



    7.5% SDS-PAGE gel.

  • Immunoprecipitation - Anti-SHP1 antibody (ab227503)
    Immunoprecipitation - Anti-SHP1 antibody (ab227503)

    SHP1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 3 µg ab227503. Western blot was performed from the immunoprecipitate using ab227503 at 1/1000 dilution. Anti-Rabbit IgG was used as a secondary reagent.

    Lane 1: HeLa whole cell lysate.

    Lane 2: Control IP in HeLa whole cell lysate with 3 μg of pre-immune rabbit IgG .

    Lane 3: ab227503 IP in HeLa whole cell lysate.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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