Human Fos B ELISA Kit (ab277436)
Key features and details
- Sensitivity: 0.09 ng/ml
- Range: 0.082 ng/ml - 20 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Fos B ELISA Kit
See all Fos B kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% General Inter-assay Sample n Mean SD CV% General -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.09 ng/ml -
Range
0.082 ng/ml - 20 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 89.62 74% - 106% Plasma 105.7 80% - 134% Cell culture media 85.23 69% - 122% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Fos B ELISA Kit (ab277436) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Fos B in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Fos B coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Fos B present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Fos B antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Fos B bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent 1 x 15ml Biotinylated Anti-Human Fos B Antibody 2 vials Anti-Human Fos B coated Microplate 1 unit Human Fos B standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
FosB interacts with Jun proteins enhancing their DNA binding activity. -
Sequence similarities
Belongs to the bZIP family. Fos subfamily.
Contains 1 bZIP domain. -
Cellular localization
Nucleus. - Information by UniProt
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Alternative names
- Activator protein 1
- AP 1
- DKFZp686C0818
see all -
Database links
- Entrez Gene: 2354 Human
- Omim: 164772 Human
- SwissProt: P53539 Human
- Unigene: 590958 Human
Images
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Human Fos B ELISA kit (ab277436) Standard curve.
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.