Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-c-Jun antibody [EP693Y] - BSA and Azide free (ab247284)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP693Y] to c-Jun - BSA and Azide free
  • Suitable for: IHC-P, ICC, WB, IP, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human, A wide range of other invertebrates

Overview

  • Product name

    Anti-c-Jun antibody [EP693Y] - BSA and Azide free
    See all c-Jun primary antibodies

  • Description

    Rabbit monoclonal [EP693Y] to c-Jun - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    PBS only lot tested.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    IHC-P
    Mouse
    IP
    Mouse
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293, MOJ/3T3 and PC-12 cell lysates;. IHC-P: Rat liver, mouse cerebrum and human cervix carcinoma tissues. ICC: NIH/3T3 and HeLa cells. ICC KO: HEK293 cells (HEK293-JUN KO cells used as a negative cell line). Flow Cyt: HEK-293 cells IP: NIH/3T3 cell lysate

  • General notes

    ab247284 is the carrier-free version of ab40766 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247284 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry - Anti-c-Jun antibody [EP693Y] - BSA and Azide free (ab247284)
    Immunocytochemistry - Anti-c-Jun antibody [EP693Y] - BSA and Azide free (ab247284)

    This data was developed using ab40766, the same antibody clone in a different buffer formulation.

    ab40766 staining c-Jun in wild-type HEK293 cells (top panel) and c-Jun knockout HEK293 cells (bottom panel). The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab40766 at 1/250 dilution and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI. Image was taken with a high-content analysis system (Perkin Elmer, Operetta CLS™).
  • Immunoprecipitation - Anti-c-Jun antibody [EP693Y] - BSA and Azide free (ab247284)
    Immunoprecipitation - Anti-c-Jun antibody [EP693Y] - BSA and Azide free (ab247284)

    This data was developed using ab40766, the same antibody clone in a different buffer formulation.
    Purified ab40766 at 1/20 dilution (1µg) immunoprecipitating c-Jun in NIH/3T3 whole cell lysate.
    Lane 1 (input): NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate 10µg
    Lane 2 (+): ab40766 + NIH/3T3 whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab40766 in NIH/3T3 whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 39 kDa

  • Western blot - Anti-c-Jun antibody [EP693Y] - BSA and Azide free (ab247284)
    Western blot - Anti-c-Jun antibody [EP693Y] - BSA and Azide free (ab247284)
    All lanes : Anti-c-Jun antibody [EP693Y] (ab40766) at 1/1000 dilution (Purified)

    Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 39 kDa
    Observed band size: 39 kDa



    This data was developed using ab40766, the same antibody clone in a different buffer formulation. 

    Blocking Buffer and concentration: 5% NFDM/TBST

  • Immunocytochemistry - Anti-c-Jun antibody [EP693Y] - BSA and Azide free (ab247284)
    Immunocytochemistry - Anti-c-Jun antibody [EP693Y] - BSA and Azide free (ab247284)

    This data was developed using ab40766, the same antibody clone in a different buffer formulation.
    Immunocytochemistry analysis of NIH/3T3 (Mouse embryonic fibroblast) cells labeling c-Jun with Purified ab247284 at 1:50 dilution (4.06 ?g/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry - Anti-c-Jun antibody [EP693Y] - BSA and Azide free (ab247284)
    Flow Cytometry - Anti-c-Jun antibody [EP693Y] - BSA and Azide free (ab247284)

    This data was developed using ab40766, the same antibody clone in a different buffer formulation.
    Flow Cytometry analysis of HEK-293 (Human embryonic kidney epithelial cell) cells labeling c-Jun with Purified ab247284 at 1:20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [EP693Y] - BSA and Azide free (ab247284)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [EP693Y] - BSA and Azide free (ab247284)

    This data was developed using ab40766, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling c-Jun with Purified ab247284 at 1:500 dilution (0.41 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [EP693Y] - BSA and Azide free (ab247284)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [EP693Y] - BSA and Azide free (ab247284)

    This data was developed using ab40766, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling c-Jun with Purified ab247284 at 1:500 dilution (0.41 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [EP693Y] - BSA and Azide free (ab247284)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [EP693Y] - BSA and Azide free (ab247284)

    This data was developed using ab40766, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervix carcinoma tissue sections labeling c-Jun with Purified ab247284 at 1:500 dilution (0.41 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Western blot - Anti-c-Jun antibody [EP693Y] - BSA and Azide free (ab247284)
    Western blot - Anti-c-Jun antibody [EP693Y] - BSA and Azide free (ab247284)
    All lanes : Anti-c-Jun antibody [EP693Y] (ab40766) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293 whole cell lysate
    Lane 2 : Jun knockout HEK-293 whole cell lysate

    Lysates/proteins at 40 µg per lane.

    Predicted band size: 39 kDa



    This data was developed using ab40766, the same antibody clone in a different buffer formulation.

    Lanes 1 - 2: Merged signal (red and green). Green - ab40766 observed at 35 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab40766 was shown to specifically react with Jun in wild-type HEK-293 cells as signal was lost in Jun knockout cells. Wild-type and Jun knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab40766 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry - Anti-c-Jun antibody [EP693Y] - BSA and Azide free (ab247284)
    Immunocytochemistry - Anti-c-Jun antibody [EP693Y] - BSA and Azide free (ab247284)
    This data was developed using ab40766, the same antibody clone in a different buffer formulation.Immunofluorescent staining of HeLa cells
    ab40766 at 1/100 dilution
  • Anti-c-Jun antibody [EP693Y] - BSA and Azide free (ab247284)
    Anti-c-Jun antibody [EP693Y] - BSA and Azide free (ab247284)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-c-Jun antibody [EP693Y] - BSA and Azide free (ab247284)

© 2021 Astana Biomed Group LLP. All rights reserved.