Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ATF1 antibody [EPR17028] - BSA and Azide free (ab240253)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17028] to ATF1 - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt, IP, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ATF1 antibody [EPR17028] - BSA and Azide free
    See all ATF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR17028] to ATF1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Mouse
    IHC-P
    Mouse
    IP
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab240253 is the carrier-free version of ab181569. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240253 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (ab240253)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (ab240253)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling ATF1 with ab181569 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on mouse liver (PMID: 11865068; PMID: 28032861). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181569).

  • Immunocytochemistry/ Immunofluorescence - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (ab240253)
    Immunocytochemistry/ Immunofluorescence - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (ab240253)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling ATF1 with ab181569 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181569).

  • Immunocytochemistry/ Immunofluorescence - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (ab240253)
    Immunocytochemistry/ Immunofluorescence - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (ab240253)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryo fibroblast cell line) cells labeling ATF1 with ab181569 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on NIH/3T3 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181569).

  • Flow Cytometry - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (ab240253)
    Flow Cytometry - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (ab240253)

    Flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling ATF1 with ab181569 at 1/150 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181569).

  • Immunoprecipitation - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (ab240253)
    Immunoprecipitation - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (ab240253)

    ATF1 was immunoprecipitated from 1 mg of mouse kidney lysate with ab181569 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab181569 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse kidney lysate 10 µg (Input). 

    Lane 2: ab181569 IP in mouse kidney lysate (+). 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab181569 in mouse kidney lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181569).

  • Anti-ATF1 antibody [EPR17028] - BSA and Azide free (ab240253)
    Anti-ATF1 antibody [EPR17028] - BSA and Azide free (ab240253)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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