Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23475-105] to C10 - BSA and Azide free
  • Suitable for: Flow Cyt, IHC-P, IHC-Fr, IP
  • Reacts with: Mouse

Overview

  • Product name

    Anti-C10 antibody [EPR23475-105] - BSA and Azide free
    See all C10 primary antibodies

  • Description

    Rabbit monoclonal [EPR23475-105] to C10 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, IHC-Fr, IPmore details
    Unsuitable for: ICC or WB

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse spleen and lung tissue. IHC-Fr: Mouse small intestine and colon tissue. Flow cyt: P388D1 cells (treated with 1ug/ml lipopolysaccharide (LPS) for 4h then add 300ng/ml Brefeldin A for 16h). IP: Mouse small intestine tissue lysate.

  • General notes

    ab275039 is the carrier-free version of ab275025. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23475-105

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)

    This data was developed using ab275025, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling C10 with ab275025 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Postive staining on mouse spleen (PMID: 15770051). The section was incubated with ab275025 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)
    Immunohistochemistry (Frozen sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)

    This data was developed using ab275025, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse small intestine tissue labeling C10 with ab275025 at 1/500 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on mouse small intestine (PMID: 7579352). The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution.

  • Flow Cytometry - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)
    Flow Cytometry - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)

    This data was developed using ab275025, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized P388D1 (Mouse lymphoma monocyte; macrophage) (treated with 1ug/ml lipopolysaccharide (LPS) for 4h then add 300ng/ml Brefeldin A for 16h) (Red) / Untreated control (Green) cells labelling C10 with ab275025 at 1/500 dilution (0.1ug) compared with a Rabbit monoclonal IgG (ab172730)  isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)
    Western blot - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)
    All lanes : Anti-C10 antibody [EPR23475-105] (ab275025) at 1/1000 dilution

    Lane 1 : His-tagged mouse C10 recombinant protein, 20ng
    Lane 2 : His-tagged mouse MIP-1 gamma (CCL9) recombinant protein (aa 22-122), 50ng

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 13 kDa



    This data was developed using ab275025, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 8 seconds.

    This product does not recognize CCL9.

  • Immunoprecipitation - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)
    Immunoprecipitation - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)

    This data was developed using ab275025, the same antibody clone in a different buffer formulation.

    C10 was immunoprecipitated from 0.35 mg Mouse small intestine tissue lysate with ab275025 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275025 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/1000 dilution.

    Lane 1: Mouse small intestine tissue lysate 10 ug

    Lane 2: ab275025 IP in Mouse small intestine tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275025 in mouse small intestine tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    The expression profile observed is consistent with what has been described in the literature (PMID: 19812544).

  • Immunohistochemistry (Frozen sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)
    Immunohistochemistry (Frozen sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)

    This data was developed using ab275025, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse colon tissue labeling C10 with ab275025 at 1/500 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on mouse colon (PMID: 7579352). The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 (2 ug/ml) dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)

    This data was developed using ab275025, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling C10 with ab275025 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Postive staining on mouse lung (PMID: 14734772). The section was incubated with ab275025 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)
    Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)

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