Mouse TCA-3 ELISA Kit (CCL1) (ab155460)
Key features and details
- Sensitivity: 30 pg/ml
- Range: 0.033 ng/ml - 8 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse TCA-3 ELISA Kit (CCL1) -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Intra-assay Inter-assay Sample n Mean SD CV% Inter-assay -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
Range
0.033 ng/ml - 8 ng/mlRecovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 91.6 77% - 103% Serum 127.7 119% - 135% Plasma 126.3 109% - 139% Assay duration
Multiple steps standard assaySpecies reactivity
Reacts with: MouseProduct overview
Abcam’s TCA-3 (CCL1) Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of TCA-3 (CCL1) in mouse serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse TCA-3 coated on a 96-well plate. Standards and samples are pipetted into the wells and TCA-3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse TCA-3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and colour develops in proportion to the amount of TCA-3 bound. The Stop Solution changes the colour from blue to yellow, and the intensity of the colour is measured at 450 nm.
Platform
MicroplateProperties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml 700X HRP-Streptavidin Concentrate 1 x 200µl Biotinylated anti-mouse TCA-3 2 vials Recombinant Mouse TCA-3 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TCA-3 Microplate (12 x 8 wells) 1 unit TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Alternative names
- C-C motif chemokine 1
- CCL1
- chemokine (C-C motif) ligand 1
see all
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml 700X HRP-Streptavidin Concentrate 1 x 200µl Biotinylated anti-mouse TCA-3 2 vials Recombinant Mouse TCA-3 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TCA-3 Microplate (12 x 8 wells) 1 unit TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
-
Alternative names
- C-C motif chemokine 1
- CCL1
- chemokine (C-C motif) ligand 1
see all
Images
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Typical Standard Curve
Representative standard curve using ab155460 - Assay Diluent B.
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Typical Standard CurveRepresentative standard curve using ab155460 - Assay Diluent D.