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Cancer Tumor biomarkers Enzymes Phosphatases

Human Cdc25C Antibody Pair - BSA and Azide free (ab244070)

Human Cdc25C Antibody Pair - BSA and Azide free (ab244070)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Human

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Overview

  • Product name

    Human Cdc25C Antibody Pair - BSA and Azide free
    See all Cdc25C kits
  • Assay type

    ELISA set
  • Range

    78.1 pg/ml - 5000 pg/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Antibody Pair can be used to quantify Human Cdc25C. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab230944), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay..


    Download SDS here.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Carrier free

    Yes
  • Components 10 x 96 tests
    Human Cdc25C Capture Antibody (unconjugated) 1 x 100µg
    Human Cdc25C Detector Antibody (unconjugated) 1 x 100µg
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Phosphatases
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Kinases/Phosphatases
    • Phospatases
  • Function

    Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity.
  • Sequence similarities

    Belongs to the MPI phosphatase family.
    Contains 1 rhodanese domain.
  • Developmental stage

    Expressed predominantly in G2 phase.
  • Post-translational
    modifications

    Phosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P30307 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • CDC 25
    • Cdc 25C
    • CDC25
    • CDC25C
    • Cell division cycle 25 homolog C
    • Cell division cycle 25C
    • Cell division cycle 25C protein
    • Dual specificity phosphatase Cdc25C
    • M phase inducer phosphatase 3
    • M-phase inducer phosphatase 3
    • Mitosis inducer CDC25
    • MPIP3
    • MPIP3_HUMAN
    • Phosphotyrosine phosphatase
    • PPP1R60
    • protein phosphatase 1, regulatory subunit 60
    see all
  • Database links

    • Entrez Gene: 995 Human
    • Omim: 157680 Human
    • SwissProt: P30307 Human
    • Unigene: 656 Human

    Images

    • Sandwich ELISA - Human Cdc25C Antibody Pair - BSA and Azide free (ab244070)
      Sandwich ELISA - Human Cdc25C Antibody Pair - BSA and Azide free (ab244070)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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