Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (ab240219)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16871] to Phosphotyrosine - BSA and Azide free
  • Suitable for: Flow Cyt, IP, Dot blot, ELISA, ICC/IF, WB
  • Reacts with: Rat

Overview

  • Product name

    Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free
    See all Phosphotyrosine primary antibodies

  • Description

    Rabbit monoclonal [EPR16871] to Phosphotyrosine - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IP, Dot blot, ELISA, ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab240219 is the carrier-free version of ab179530. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240219 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • ELISA - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (ab240219)
    ELISA - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (ab240219)

    Serially diluted ab179530 was bound to immobilised phospho- or control peptides (STAT1 (phospho S727), STAT1 control, STAT5 (phospho T694), STAT5 control); 1 microgram per mL).

    The antibody was detected by Goat anti-Rabbit HRPO and signal was developed by TMB substrate.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179530).

  • Dot Blot - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (ab240219)
    Dot Blot - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (ab240219)

    Dot blot analysis of INSR/IGF-1R (pY1009) phospho peptide (lane 1) and INSR/IGF-1R non-phospho peptide (lane 2) labelling Phosphotyrosine with ab179530 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179530).

  • Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (ab240219)
    Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (ab240219)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C2C12 (Mouse myoblast cell line) cells labeling Phosphotyrosine with ab179530 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Cytoplasm staining on C2C12 cells is observed. The expression increased after treatment with H2O2 (2mM) for 10 minutes. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:-
    -ve control 1: - ab179530 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: - ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179530).

  • ELISA - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (ab240219)
    ELISA - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (ab240219)

    ELISA analysis of various antigens (1 µg/ml) using ab179530 at 1/6400 dilution followed by Alkaline Phosphatase-conjugated Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

    S/N = signal-to-noise ratio of phospho- versus nonphospho-peptides.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179530).

  • Flow Cytometry - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (ab240219)
    Flow Cytometry - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (ab240219)

    Flow cytometric analysis of 2% paraformaldehyde-fixed Pervanadate (50mM, 30min.) treated (orange)/untreated (red) A431 (Human epidermoid carcinoma) cells labeling Phosphotyrosine with ab179530 at 1/160 dilution compared with a rabbit monoclonal IgG control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179530).

  • Immunoprecipitation - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (ab240219)
    Immunoprecipitation - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (ab240219)

    Phosphotyrosine was immunoprecipitated from 1mg of A431 (Human epidermoid carcinoma) whole cell extract treated with 1mM pervanadate for 30 minutes with ab179530 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab179530 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: A431 treated with 1mM pervanadate for 30 minutes whole cell extract. Lane 2: PBS instead of A431 whole cell extract.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Multiple bands represent phosph-tyrosine containing proteins precipitated and detected by ab179530.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179530).

  • Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (ab240219)
    Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (ab240219)

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