Anti-PTEN (phospho S380) antibody [EP2138Y] (ab76431)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2138Y] to PTEN (phospho S380)
- Suitable for: WB, Dot blot
- Reacts with: Mouse, Human
Overview
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Product name
Anti-PTEN (phospho S380) antibody [EP2138Y]
See all PTEN primary antibodies -
Description
Rabbit monoclonal [EP2138Y] to PTEN (phospho S380) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PTEN (phospho S38). The exact sequence is proprietary.
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Positive control
- WB: NIH3T3 cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP2138Y -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-PTEN (phospho S380) antibody [EP2138Y] (ab76431) at 1/2000 dilution
Lane 1 : NIH/3T3 whole cell lysate - treated with alkaline phosphatase
Lane 2 : NIH/3T3 whole cell lysate - untreated
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 47 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking and dilution buffer: 5% NFDM/TBST.
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Dot blot analysis of PTEN (pS380) phospho peptide (lane 1) and PTEN non-phospho peptide (lane 2) labelling PTEN (phospho S380) with ab76431 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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