Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PPP2R5D antibody [EPR15617] (ab188323)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15617] to PPP2R5D
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF, IP
  • Knockout validated
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-PPP2R5D antibody [EPR15617]
    See all PPP2R5D primary antibodies

  • Description

    Rabbit monoclonal [EPR15617] to PPP2R5D

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A431, MCF7, C6, HepG2, Jurkat, HeLa, 293T cell lysate. IHC-P: Human brain tissue. IP: HEK293 cells. Flow Cyt: HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PPP2R5D antibody [EPR15617] (ab188323)
    Western blot - Anti-PPP2R5D antibody [EPR15617] (ab188323)
    All lanes : Anti-PPP2R5D antibody [EPR15617] (ab188323) at 1/10000 dilution

    Lane 1 : Wild-type A431 cell lysate
    Lane 2 : PPP2R5D knockout A431 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 70 kDa
    Observed band size: 60-65 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 4: Merged signal (red and green). Green - ab188323 observed at 60-65 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab188323 was shown to react with PPP2R5D in wild-type A431 cells in western blot with loss of signal observed in PPP2R5D knockout sample. Wild-type and PPP2R5D knockout A431 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab188323 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP2R5D antibody [EPR15617] (ab188323)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP2R5D antibody [EPR15617] (ab188323)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling PPP2R5D using ab188323 at 1/100 dilution. A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-PPP2R5D antibody [EPR15617] (ab188323)
    Western blot - Anti-PPP2R5D antibody [EPR15617] (ab188323)
    All lanes : Anti-PPP2R5D antibody [EPR15617] (ab188323) at 1/100000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 70 kDa
    Observed band size: 70 kDa



    Based on the sequence analysis, ab188323 recognizes 3 isoforms with the predicted MWs of 58KDa, 66KDa and 70KDa, respectively.

  • Immunoprecipitation - Anti-PPP2R5D antibody [EPR15617] (ab188323)
    Immunoprecipitation - Anti-PPP2R5D antibody [EPR15617] (ab188323)

    Immunoprecipitation analysis of 293T cell lysate labeling PPP2R5D using ab188323 at 1/50 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1500 was used as secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-PPP2R5D antibody [EPR15617] (ab188323)
    Immunocytochemistry/ Immunofluorescence - Anti-PPP2R5D antibody [EPR15617] (ab188323)

    Immunofluorescence analysis of HepG2 cells labeling PPP2R5D using ab188323 at 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% Paraformaldehyde. Counterstain: DAPI.

  • Flow Cytometry - Anti-PPP2R5D antibody [EPR15617] (ab188323)
    Flow Cytometry - Anti-PPP2R5D antibody [EPR15617] (ab188323)

    Flow cytometry analysis of HeLa cells labeling PPP2R5D using ab188323 at 1/40 dilution (pink). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG (green).

  • Anti-PPP2R5D antibody [EPR15617] (ab188323)
    Anti-PPP2R5D antibody [EPR15617] (ab188323)

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