Anti-PPP2R5D antibody (ab72303)
Key features and details
- Rabbit polyclonal to PPP2R5D
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PPP2R5D antibody
See all PPP2R5D primary antibodies -
Description
Rabbit polyclonal to PPP2R5D -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region between residues 1 and 50 of human PPP2R5D (NP_006236.1).
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Positive control
- Whole cell lysate from HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PPP2R5D antibody (ab72303)All lanes : Anti-PPP2R5D antibody (ab72303) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Developed using the ECL technique.
Predicted band size: 67 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds
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Immunoprecipitation - Anti-PPP2R5D antibody (ab72303)Detection of Human PPP2R5D by Immunoprecipitation in whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72303 at 3 µg/mg lysate for IP and at 0.1 µg/ml for subsequent Western blot detection.
Detection: Chemiluminescence with an exposure time of 10 seconds. seconds
