HRP Anti-PTEN antibody [EPR9941-2] (ab202358)
![HRP Anti-PTEN antibody [EPR9941-2] (ab202358)](https://www.abcam.com/ps/products/202/ab202358/Images/ab202358-333747-anti-pten-antibody-epr9941-2-hrp-western-blot.jpg "HRP Anti-PTEN antibody [EPR9941-2] (ab202358)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR9941-2] to PTEN
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-PTEN antibody [EPR9941-2]
See all PTEN primary antibodies -
Description
HRP Rabbit monoclonal [EPR9941-2] to PTEN -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Recombinant fragment within Human PTEN aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P60484 -
Positive control
- WB: HeLa, MCF7 and HEK293T whole cell lysates.
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General notes
Alternative versions available:
Anti-PTEN antibody [EPR9941-2] (ab170941) - Knockout validatedOur RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9941-2 -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-PTEN antibody [EPR9941-2] (ab202358)All lanes : HRP Anti-PTEN antibody [EPR9941-2] (ab202358) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : PTEN knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 16 minutesab202358 was shown to specifically react with PTEN in wild-type HAP1 cells as signal was lost in PTEN knockout cells. Wild-type and PTEN knockout samples were subjected to SDS-PAGE. Ab202358 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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![Western blot - HRP Anti-PTEN antibody [EPR9941-2] (ab202358)](https://www.abcam.com/ps/products/202/ab202358/Images/ab202358-249290-anti-pten-antibody-epr9941-2-hrp-western-blot.jpg)
Western blot - HRP Anti-PTEN antibody [EPR9941-2] (ab202358)All lanes : HRP Anti-PTEN antibody [EPR9941-2] (ab202358) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab202358 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-PTEN antibody [EPR9941-2] (ab202358)](https://www.abcam.com/ps/products/202/ab202358/Images/ab202358-4-benefits-of-recombinant-antibodies.png)
HRP Anti-PTEN antibody [EPR9941-2] (ab202358)
