Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PTEN antibody [EPR23729-4] - BSA and Azide free (ab273629)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23729-4] to PTEN - BSA and Azide free
  • Suitable for: WB, Flow Cyt, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PTEN antibody [EPR23729-4] - BSA and Azide free
    See all PTEN primary antibodies

  • Description

    Rabbit monoclonal [EPR23729-4] to PTEN - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, IPmore details
    Unsuitable for: ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild-type HAP1 whole cell lysate; MCF7, RAW 264.7, PC-12, NIH/3T3, Neuro 2a and HeLa whole cell lysates. Flow Cyt: Wild-type HAP1, MCF7 and NIH/3T3 cells. IP: MCF7 and NIH/3T3 whole cell lysates.

  • General notes

    ab273629 is the carrier-free version of ab260011. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PTEN antibody [EPR23729-4] - BSA and Azide free (ab273629)
    Western blot - Anti-PTEN antibody [EPR23729-4] - BSA and Azide free (ab273629)
    All lanes : Anti-PTEN antibody [EPR23729-4] (ab260011) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : PTEN knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 47 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 59 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260011).

  • Flow Cytometry - Anti-PTEN antibody [EPR23729-4] - BSA and Azide free (ab273629)
    Flow Cytometry - Anti-PTEN antibody [EPR23729-4] - BSA and Azide free (ab273629)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized Parental HAP1 (Wildtype control Human chronic myelogenous leukemia near-haploid cell line) (Right) / PTEN KO HAP1 (Left) cells labelling PTEN with ab260011 at 1/600 dilution (0.1ug) (Red)  compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260011).

  • Immunoprecipitation - Anti-PTEN antibody [EPR23729-4] - BSA and Azide free (ab273629)
    Immunoprecipitation - Anti-PTEN antibody [EPR23729-4] - BSA and Azide free (ab273629)

    PTEN was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast), whole cell lysate with ab260011 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab260011 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate 10 ug

    Lane 2: ab260011 IP in NIH/3T3 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab260011 in NIH/3T3 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260011).

  • Flow Cytometry - Anti-PTEN antibody [EPR23729-4] - BSA and Azide free (ab273629)
    Flow Cytometry - Anti-PTEN antibody [EPR23729-4] - BSA and Azide free (ab273629)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling PTEN with ab260011 at 1/60 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260011).

  • Flow Cytometry - Anti-PTEN antibody [EPR23729-4] - BSA and Azide free (ab273629)
    Flow Cytometry - Anti-PTEN antibody [EPR23729-4] - BSA and Azide free (ab273629)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) cells labelling PTEN with ab260011 at 1/60 dilution (1ug) (Red)  compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260011).

  • Immunoprecipitation - Anti-PTEN antibody [EPR23729-4] - BSA and Azide free (ab273629)
    Immunoprecipitation - Anti-PTEN antibody [EPR23729-4] - BSA and Azide free (ab273629)

    PTEN was immunoprecipitated from 0.35 mg MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate with ab260011 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab260011 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate 10 ug

    Lane 2: ab260011 IP in MCF7 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab260011 in MCF7 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260011).

  • Anti-PTEN antibody [EPR23729-4] - BSA and Azide free (ab273629)
    Anti-PTEN antibody [EPR23729-4] - BSA and Azide free (ab273629)

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