Antibodies, Proteins, Kits and Reagents for Life Science

361 843 ₸ Product size

100 µl 361 843 ₸

Order now and get it on Tuesday March 09, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9941] to PTEN
Suitable for: WB
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-PTEN antibody [EPR9941]
See all PTEN primary antibodies
Description

Rabbit monoclonal [EPR9941] to PTEN
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Mouse

Human

See all applications and species data
Immunogen

Recombinant full length protein corresponding to Human PTEN.
Positive control
- HeLa, MCF7, 293T and A431 cell lysates; Human breast carcinoma tissue and Human colon tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR9941
Isotype

IgG
Research areas

Western blot - Anti-PTEN antibody [EPR9941] (ab154812)

All lanes : Anti-PTEN antibody [EPR9941] (ab154812) at 1/10000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PTEN knockout HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 47 kDa
Observed band size: 47 kDa

Lanes 1- 2: Merged signal (red and green). Green - ab154812 observed at 47 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab154812 was shown to react with PTEN in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab255419 (knockout cell lysate ab263829) was used. Wild-type HeLa and PTEN knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab154812 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-PTEN antibody [EPR9941] (ab154812)

All lanes : Anti-PTEN antibody [EPR9941] (ab154812) at 1/1000 dilution

Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : PTEN knockout HAP1 cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 47 kDa

Lanes 1 and 2: Merged signal (red and green). Green - ab154812 observed at 51 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab154812 was shown to specifically recognize PTEN in wild-type HAP1 cells. No band was observed when PTEN knockout samples were used. Wild-type and PTEN knockout samples were subjected to SDS-PAGE, ab154812 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.
Western blot - Anti-PTEN antibody [EPR9941] (ab154812)

All lanes : Anti-PTEN antibody [EPR9941] (ab154812) at 1/1000 dilution

Lane 1 : Mouse brain Lysate
Lane 2 : Rat brain Lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

Predicted band size: 47 kDa
Observed band size: 54 kDa
why is the actual band size different from the predicted?
Western blot - Anti-PTEN antibody [EPR9941] (ab154812)

All lanes : Anti-PTEN antibody [EPR9941] (ab154812) at 1/1000 dilution

Lane 1 : HeLa cell Lysate
Lane 2 : Neuro-2a cell Lysate
Lane 3 : Rat spleen cell Lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

Predicted band size: 47 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
Western blot - Anti-PTEN antibody [EPR9941] (ab154812)

All lanes : Anti-PTEN antibody [EPR9941] (ab154812) at 1/1000 dilution (unpurified)

Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : A431 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 47 kDa
Anti-PTEN antibody [EPR9941] (ab154812)

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