Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PTEN (phospho S380) antibody [EP2138Y] - BSA and Azide free (ab247473)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2138Y] to PTEN (phospho S380) - BSA and Azide free
  • Suitable for: Dot blot, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PTEN (phospho S380) antibody [EP2138Y] - BSA and Azide free
    See all PTEN primary antibodies

  • Description

    Rabbit monoclonal [EP2138Y] to PTEN (phospho S380) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247473 is the carrier-free version of ab76431. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247473 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PTEN (phospho S380) antibody [EP2138Y] - BSA and Azide free (ab247473)
    Western blot - Anti-PTEN (phospho S380) antibody [EP2138Y] - BSA and Azide free (ab247473)
    All lanes : Anti-PTEN (phospho S380) antibody [EP2138Y] (ab76431) at 1/2000 dilution

    Lane 1 : NIH/3T3 whole cell lysate - treated with alkaline phosphatase
    Lane 2 : NIH/3T3 whole cell lysate - untreated

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 47 kDa
    Observed band size: 54 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    This data was developed using ab76431, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Dot Blot - Anti-PTEN (phospho S380) antibody [EP2138Y] - BSA and Azide free (ab247473)
    Dot Blot - Anti-PTEN (phospho S380) antibody [EP2138Y] - BSA and Azide free (ab247473)
    This data was developed using ab76431, the same antibody clone in a different buffer formulation.Dot blot analysis of PTEN (pS380) phospho peptide (lane 1) and PTEN non-phospho peptide (lane 2) labelling PTEN (phospho S380) with ab76431 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).Blocking and dilution buffer: 5% NFDM/TBST.Exposure time: 3 minutes.
  • Anti-PTEN (phospho S380) antibody [EP2138Y] - BSA and Azide free (ab247473)
    Anti-PTEN (phospho S380) antibody [EP2138Y] - BSA and Azide free (ab247473)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.