Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PDE10A antibody [EPR22383] - BSA and Azide free (ab245198)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22383] to PDE10A - BSA and Azide free
  • Suitable for: IHC-Fr, IP, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PDE10A antibody [EPR22383] - BSA and Azide free
    See all PDE10A primary antibodies

  • Description

    Rabbit monoclonal [EPR22383] to PDE10A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Fr, IP, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa whole cell lysate; Wild-type HAP1 whole cell lysate. IHC-Fr: Mouse and rat brain tissues. IP: HeLa whole cell lysate.

  • General notes

    ab245198 is the carrier-free version of ab227829.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PDE10A antibody [EPR22383] - BSA and Azide free (ab245198)
    Western blot - Anti-PDE10A antibody [EPR22383] - BSA and Azide free (ab245198)
    All lanes : Anti-PDE10A antibody [EPR22383] (ab227829) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : PDE10A knockout HAP1 whole cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 88 kDa
    Observed band size: 88 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    ab227829 was shown to specifically react with PDE10A in wild-type HAP1 cells as signal was lost in PDE10A knockout cells. Wild-type and PDE10A knockout samples were subjected to SDS-PAGE. ab227829 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging.

    The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227829).

  • Immunohistochemistry (Frozen sections) - Anti-PDE10A antibody [EPR22383] - BSA and Azide free (ab245198)
    Immunohistochemistry (Frozen sections) - Anti-PDE10A antibody [EPR22383] - BSA and Azide free (ab245198)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse brain tissue labeling PDE10A with ab227829 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on corpus striatum in mouse brain (PMID:16864896) is observed. Counterstained with DAPI (blue).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227829).

  • Immunohistochemistry (Frozen sections) - Anti-PDE10A antibody [EPR22383] - BSA and Azide free (ab245198)
    Immunohistochemistry (Frozen sections) - Anti-PDE10A antibody [EPR22383] - BSA and Azide free (ab245198)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse brain tissue labeling PDE10A with ab227829 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on mouse caudate-putamen and globus pallidus in mouse brain (PMID:16864896; PMID:28115486) is observed. Counterstained with DAPI (blue).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227829).

  • Immunohistochemistry (Frozen sections) - Anti-PDE10A antibody [EPR22383] - BSA and Azide free (ab245198)
    Immunohistochemistry (Frozen sections) - Anti-PDE10A antibody [EPR22383] - BSA and Azide free (ab245198)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat brain tissue labeling PDE10A with ab227829 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on corpus striatum in rat brain (PMID:16864896) is observed. Counterstained with DAPI (blue).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227829).

  • Immunoprecipitation - Anti-PDE10A antibody [EPR22383] - BSA and Azide free (ab245198)
    Immunoprecipitation - Anti-PDE10A antibody [EPR22383] - BSA and Azide free (ab245198)

    PDE10A was immunoprecipitated from 0.35 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab227829 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab227829 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
    Lane 1: HeLa whole cell lysate 10 µg (Input).
    Lane 2: ab227829 IP in HeLa whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab227829 in HeLa whole cell lysate.
    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227829).

  • Immunohistochemistry (Frozen sections) - Anti-PDE10A antibody [EPR22383] - BSA and Azide free (ab245198)
    Immunohistochemistry (Frozen sections) - Anti-PDE10A antibody [EPR22383] - BSA and Azide free (ab245198)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat kidney tissue labeling PDE10A with ab227829 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Negative control: No staining on rat kidney (PMID:16864896). Counterstained with DAPI (blue).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227829).

  • Immunohistochemistry (Frozen sections) - Anti-PDE10A antibody [EPR22383] - BSA and Azide free (ab245198)
    Immunohistochemistry (Frozen sections) - Anti-PDE10A antibody [EPR22383] - BSA and Azide free (ab245198)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling PDE10A with ab227829 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Negative control: No staining on mouse kidney (PMID:16864896). Counterstained with DAPI (blue).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227829).

  • Anti-PDE10A antibody [EPR22383] - BSA and Azide free (ab245198)
    Anti-PDE10A antibody [EPR22383] - BSA and Azide free (ab245198)

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