Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Tuesday March 02, 2021

Anti-PDE10A antibody [EPR22383] (ab227829)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22383] to PDE10A
Suitable for: WB, IHC-Fr, IP
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-PDE10A antibody [EPR22383]
See all PDE10A primary antibodies
Description

Rabbit monoclonal [EPR22383] to PDE10A
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse Rat
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa whole cell lysate; Wild-type HAP1 whole cell lysate; Human, mouse and rat brain lysates; Mouse testis lysate. IHC-Fr: Mouse and rat brain tissues. IP: HeLa whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22383
Isotype

IgG
Research areas

Western blot - Anti-PDE10A antibody [EPR22383] (ab227829)

All lanes : Anti-PDE10A antibody [EPR22383] (ab227829) at 1/1000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : PDE10A knockout HAP1 whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 88 kDa
Observed band size: 88 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

ab227829 was shown to specifically react with PDE10A in wild-type HAP1 cells as signal was lost in PDE10A knockout cells. Wild-type and PDE10A knockout samples were subjected to SDS-PAGE. ab227829 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging.

The blot was developed on a BIO-RAD^® ChemiDoc™ MP instrument using the ECL technique.
Immunohistochemistry (Frozen sections) - Anti-PDE10A antibody [EPR22383] (ab227829)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse brain tissue labeling PDE10A with ab227829 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on corpus striatum in mouse brain (PMID:16864896) is observed. Counterstained with DAPI (blue).

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Immunohistochemistry (Frozen sections) - Anti-PDE10A antibody [EPR22383] (ab227829)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse brain tissue labeling PDE10A with ab227829 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on mouse caudate-putamen and globus pallidus in mouse brain (PMID:16864896; PMID:28115486) is observed. Counterstained with DAPI (blue).

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Immunohistochemistry (Frozen sections) - Anti-PDE10A antibody [EPR22383] (ab227829)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat brain tissue labeling PDE10A with ab227829 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on corpus striatum in rat brain (PMID:16864896) is observed. Counterstained with DAPI (blue).

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Western blot - Anti-PDE10A antibody [EPR22383] (ab227829)

All lanes : Anti-PDE10A antibody [EPR22383] (ab227829) at 1/1000 dilution

Lane 1 : Human brain lysate
Lane 2 : Mouse brain lysate
Lane 3 : Mouse testis lysate
Lane 4 : Rat brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 88 kDa
Observed band size: 88 kDa

Exposure time : Lane 1: 3 minutes; Lane 2-3: 3 seconds; Lane 4: 26 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Frozen sections) - Anti-PDE10A antibody [EPR22383] (ab227829)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat kidney tissue labeling PDE10A with ab227829 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Negative control: No staining on rat kidney (PMID:16864896). Counterstained with DAPI (blue).

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Immunohistochemistry (Frozen sections) - Anti-PDE10A antibody [EPR22383] (ab227829)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling PDE10A with ab227829 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Negative control: No staining on mouse kidney (PMID:16864896). Counterstained with DAPI (blue).

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Immunoprecipitation - Anti-PDE10A antibody [EPR22383] (ab227829)

PDE10A was immunoprecipitated from 0.35 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab227829 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab227829 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 10 µg (Input).
Lane 2: ab227829 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab227829 in HeLa whole cell lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Anti-PDE10A antibody [EPR22383] (ab227829)

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