Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PDE10A antibody [EPR22383] (ab227829)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22383] to PDE10A
  • Suitable for: WB, IHC-Fr, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PDE10A antibody [EPR22383]
    See all PDE10A primary antibodies

  • Description

    Rabbit monoclonal [EPR22383] to PDE10A

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    Rat
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa whole cell lysate; Wild-type HAP1 whole cell lysate; Human, mouse and rat brain lysates; Mouse testis lysate. IHC-Fr: Mouse and rat brain tissues. IP: HeLa whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22383

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-PDE10A antibody [EPR22383] (ab227829)
    Western blot - Anti-PDE10A antibody [EPR22383] (ab227829)
    All lanes : Anti-PDE10A antibody [EPR22383] (ab227829) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : PDE10A knockout HAP1 whole cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 88 kDa
    Observed band size: 88 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    ab227829 was shown to specifically react with PDE10A in wild-type HAP1 cells as signal was lost in PDE10A knockout cells. Wild-type and PDE10A knockout samples were subjected to SDS-PAGE. ab227829 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging.

    The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

  • Immunohistochemistry (Frozen sections) - Anti-PDE10A antibody [EPR22383] (ab227829)
    Immunohistochemistry (Frozen sections) - Anti-PDE10A antibody [EPR22383] (ab227829)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse brain tissue labeling PDE10A with ab227829 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on corpus striatum in mouse brain (PMID:16864896) is observed. Counterstained with DAPI (blue).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Immunohistochemistry (Frozen sections) - Anti-PDE10A antibody [EPR22383] (ab227829)
    Immunohistochemistry (Frozen sections) - Anti-PDE10A antibody [EPR22383] (ab227829)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse brain tissue labeling PDE10A with ab227829 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on mouse caudate-putamen and globus pallidus in mouse brain (PMID:16864896; PMID:28115486) is observed. Counterstained with DAPI (blue).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Immunohistochemistry (Frozen sections) - Anti-PDE10A antibody [EPR22383] (ab227829)
    Immunohistochemistry (Frozen sections) - Anti-PDE10A antibody [EPR22383] (ab227829)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat brain tissue labeling PDE10A with ab227829 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on corpus striatum in rat brain (PMID:16864896) is observed. Counterstained with DAPI (blue).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Western blot - Anti-PDE10A antibody [EPR22383] (ab227829)
    Western blot - Anti-PDE10A antibody [EPR22383] (ab227829)
    All lanes : Anti-PDE10A antibody [EPR22383] (ab227829) at 1/1000 dilution

    Lane 1 : Human brain lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Mouse testis lysate
    Lane 4 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 88 kDa
    Observed band size: 88 kDa



    Exposure time : Lane 1: 3 minutes; Lane 2-3: 3 seconds; Lane 4: 26 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Frozen sections) - Anti-PDE10A antibody [EPR22383] (ab227829)
    Immunohistochemistry (Frozen sections) - Anti-PDE10A antibody [EPR22383] (ab227829)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat kidney tissue labeling PDE10A with ab227829 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Negative control: No staining on rat kidney (PMID:16864896). Counterstained with DAPI (blue).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Immunohistochemistry (Frozen sections) - Anti-PDE10A antibody [EPR22383] (ab227829)
    Immunohistochemistry (Frozen sections) - Anti-PDE10A antibody [EPR22383] (ab227829)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling PDE10A with ab227829 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Negative control: No staining on mouse kidney (PMID:16864896). Counterstained with DAPI (blue).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Immunoprecipitation - Anti-PDE10A antibody [EPR22383] (ab227829)
    Immunoprecipitation - Anti-PDE10A antibody [EPR22383] (ab227829)

    PDE10A was immunoprecipitated from 0.35 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab227829 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab227829 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
    Lane 1: HeLa whole cell lysate 10 µg (Input).
    Lane 2: ab227829 IP in HeLa whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab227829 in HeLa whole cell lysate.
    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 3 minutes.

  • Anti-PDE10A antibody [EPR22383] (ab227829)
    Anti-PDE10A antibody [EPR22383] (ab227829)

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