Human PDE3B knockout MCF7 cell lysate (ab258574)
Overview
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Product name
Human PDE3B knockout MCF7 cell lysate
See all PDE3B kits -
Product overview
Knockout cell lysate achieved by CRISPR/Cas9. -
Parental Cell Line
MCF7 -
Organism
Human -
Mutation description
Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and 5 bp deletion in exon1. -
Passage number
Knockout validation
Sanger SequencingReconstitution notes
To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.*Usage of SDS sample buffer is not recommended with these lyophilized lysates.
Notes
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version - found here. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
See here for more information on knockout cell lysates.Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab263657 - Human PDE3B knockout MCF7 cell lysate 1 x 100µg ab263912 - Human wild-type MCF7 cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
Disease
Adenocarcinoma
Target
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Function
Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. May play a role in fat metabolism. -
Tissue specificity
Abundant in adipose tissues. -
Sequence similarities
Belongs to the cyclic nucleotide phosphodiesterase family. PDE3 subfamily. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- 5''-cyclic phosphodiesterase B
- Adipocyte cAMP phosphodiesterase cGMP inhibited
- AI847709
see all
Properties
-
Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab263657 - Human PDE3B knockout MCF7 cell lysate 1 x 100µg ab263912 - Human wild-type MCF7 cell lysate 1 x 100µg -
Research areas
-
Cell type
epithelial -
Disease
Adenocarcinoma