Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Dopamine Receptor D1 antibody [EP1560Y] - BSA and Azide free (ab170146)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1560Y] to Dopamine Receptor D1 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Dopamine Receptor D1 antibody [EP1560Y] - BSA and Azide free
    See all Dopamine Receptor D1 primary antibodies

  • Description

    Rabbit monoclonal [EP1560Y] to Dopamine Receptor D1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Dopamine Receptor D1 aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P21728

  • General notes

    Ab170146 is the carrier-free version of ab81296. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab170146 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] - BSA and Azide free (ab170146)
    Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] - BSA and Azide free (ab170146)
    All lanes : Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/1000 dilution (purified)

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse kidney tissue lysate
    Lane 3 : Rat brain tissue lysate
    Lane 4 : Rat kidney tissue lysate
    Lane 5 : Guinea pig brain tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Rabbit monoclonal [EP1560Y] to Dopamine Receptor D1 (ab81296) at 1/20000 dilution

    Predicted band size: 49 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab81296, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] - BSA and Azide free (ab170146)
    Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] - BSA and Azide free (ab170146)
    Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/5000 dilution (purified) + SH-SY5Y whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 49 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?



    This data was developed using ab81296, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] - BSA and Azide free (ab170146)
    Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] - BSA and Azide free (ab170146)
    Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/1000 dilution (purified) + Human fetal kidney tissue lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 49 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?



    This data was developed using ab81296, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] - BSA and Azide free (ab170146)
    Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] - BSA and Azide free (ab170146)
    Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/20000 dilution (unpurified) + SH-SY5Y cell lysate at 10 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 49 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?



    This data was developed using ab81296, the same antibody clone in a different buffer formulation.

     

  • Anti-Dopamine Receptor D1 antibody [EP1560Y] - BSA and Azide free (ab170146)
    Anti-Dopamine Receptor D1 antibody [EP1560Y] - BSA and Azide free (ab170146)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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