Anti-Dopamine Receptor D1 antibody [EP1560Y] - BSA and Azide free (ab170146)
![Anti-Dopamine Receptor D1 antibody [EP1560Y] - BSA and Azide free (ab170146)](https://www.abcam.com/ps/products/170/ab170146/Images/ab170146-410652-anti-dopamine-receptor-d1-antibody-ep1560y-bsa-and-azide-free-western-blot.jpg "Anti-Dopamine Receptor D1 antibody [EP1560Y] - BSA and Azide free (ab170146)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1560Y] to Dopamine Receptor D1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Dopamine Receptor D1 antibody [EP1560Y] - BSA and Azide free
See all Dopamine Receptor D1 primary antibodies -
Description
Rabbit monoclonal [EP1560Y] to Dopamine Receptor D1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human Dopamine Receptor D1 aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P21728 -
General notes
Ab170146 is the carrier-free version of ab81296. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab170146 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1560Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] - BSA and Azide free (ab170146)All lanes : Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/1000 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Rat brain tissue lysate
Lane 4 : Rat kidney tissue lysate
Lane 5 : Guinea pig brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Rabbit monoclonal [EP1560Y] to Dopamine Receptor D1 (ab81296) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?This data was developed using ab81296, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] - BSA and Azide free (ab170146)](https://www.abcam.com/ps/products/170/ab170146/Images/ab170146-407967-anti-dopamine-receptor-d1-antibody-ep1560y-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] - BSA and Azide free (ab170146)Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/5000 dilution (purified) + SH-SY5Y whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?This data was developed using ab81296, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] - BSA and Azide free (ab170146)](https://www.abcam.com/ps/products/170/ab170146/Images/ab170146-405647-anti-dopamine-receptor-d1-antibody-ep1560y-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] - BSA and Azide free (ab170146)Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/1000 dilution (purified) + Human fetal kidney tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?This data was developed using ab81296, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] - BSA and Azide free (ab170146)](https://www.abcam.com/ps/products/170/ab170146/Images/ab170146-397976-anti-dopamine-receptor-d1-antibody-ep1560y-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] - BSA and Azide free (ab170146)Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/20000 dilution (unpurified) + SH-SY5Y cell lysate at 10 µg
Secondary
HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 49 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?This data was developed using ab81296, the same antibody clone in a different buffer formulation.
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![Anti-Dopamine Receptor D1 antibody [EP1560Y] - BSA and Azide free (ab170146)](https://www.abcam.com/ps/products/170/ab170146/Images/ab170146-5-benefits-of-recombinant-antibodies.png)
Anti-Dopamine Receptor D1 antibody [EP1560Y] - BSA and Azide free (ab170146)
