Anti-Dopamine Receptor D1 antibody (ab40653)
Key features and details
- Rabbit polyclonal to Dopamine Receptor D1
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Dopamine Receptor D1 antibody
See all Dopamine Receptor D1 primary antibodies -
Description
Rabbit polyclonal to Dopamine Receptor D1 -
Host species
Rabbit -
Specificity
This antibody shows reactivity with: D1 Dopamine Receptor (9-21) 100%; D1 Dopamine Receptor 90%; D2 Dopamine Receptor (272-282) 0%; D2 Dopamine Receptor 0%; D3 Dopamine Receptor (2-10) 0%; D3 Dopamine Receptor 0%; D4 Dopamine Receptor (176-185) 0%; D4 Dopamine Receptor 0%; D5 Dopamine Receptor (23-35) 0%; and D5 Dopamine Receptor 0%. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Cow, Pig, Macaque monkey, Rhesus monkey
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Immunogen
Synthetic peptide:
MDGTGLVVERDFS
linked to a carrier protein by a carboxyl terminal Cysteine residue linker. Sequence corresponds to amino acids 9-21 of Human Dopamine Receptor D1.
Properties
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Form
Lyophilized:Reconstitute with 0.1ml of PBS which contains 10 mg/ml BSA. -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody (ab40653)ab40653 (1:500) staining Dopamine Receptor D1 in human cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cytoplasmic and membrane regions in the fibrous glial cells.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
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Western blot - Anti-Dopamine Receptor D1 antibody (ab40653)All lanes : Anti-Dopamine Receptor D1 antibody (ab40653) at 1/1000 dilution
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Rat Cortex Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 49 kDa
Additional bands at: 42 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
