Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1560Y] to Dopamine Receptor D1
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Guinea pig, Human

Overview

  • Product name

    Anti-Dopamine Receptor D1 antibody [EP1560Y]
    See all Dopamine Receptor D1 primary antibodies

  • Description

    Rabbit monoclonal [EP1560Y] to Dopamine Receptor D1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Dopamine Receptor D1 aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P21728

  • Positive control

    • WB: SH-SY5Y cell lysate, human fetal kidney, guinea pig brain, mouse and rat brain and kidney tissue lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EP1560Y

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)
    Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)
    All lanes : Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/1000 dilution (purified)

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse kidney tissue lysate
    Lane 3 : Rat brain tissue lysate
    Lane 4 : Rat kidney tissue lysate
    Lane 5 : Guinea pig brain tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Rabbit monoclonal [EP1560Y] to Dopamine Receptor D1 (ab81296) at 1/20000 dilution

    Predicted band size: 49 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM /TBST.

  • Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)
    Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)
    Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/5000 dilution (purified) + SH-SY5Y whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 49 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM /TBST.

  • Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)
    Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)
    Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/1000 dilution (purified) + Human fetal kidney tissue lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 49 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM /TBST.

  • Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)
    Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)
    Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/20000 dilution (unpurified) + SH-SY5Y cell lysate at 10 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 49 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?

  • Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)
    Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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