Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RBMX2 antibody [EPR14394] (ab180940)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14394] to RBMX2
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-RBMX2 antibody [EPR14394]
    See all RBMX2 primary antibodies

  • Description

    Rabbit monoclonal [EPR14394] to RBMX2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2, Hela and HT-1376; Human ovarian carcinoma and mouse testis tissues; HepG2 and Hela cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR14394

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-RBMX2 antibody [EPR14394] (ab180940)
    Immunocytochemistry/ Immunofluorescence - Anti-RBMX2 antibody [EPR14394] (ab180940)

    Immunofluorescent analysis of 4% paraformaldehyde fixed HepG2 cell line staining RBMX2 using ab180940 at a 1/100 dilution. Goat anti rabbit IgG (Dylight 488), at a dilution of 1/200, was used as a secondary antibody. Counter stain: Dapi (blue).

  • Western blot - Anti-RBMX2 antibody [EPR14394] (ab180940)
    Western blot - Anti-RBMX2 antibody [EPR14394] (ab180940)
    All lanes : Anti-RBMX2 antibody [EPR14394] (ab180940) at 1/10000 dilution

    Lane 1 : HepG2
    Lane 2 : HeLa

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa



    Blocking buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-RBMX2 antibody [EPR14394] (ab180940)
    Western blot - Anti-RBMX2 antibody [EPR14394] (ab180940)
    Anti-RBMX2 antibody [EPR14394] (ab180940) at 1/2000 dilution + HT-1376 at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa



    Blocking buffer and concentration: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMX2 antibody [EPR14394] (ab180940)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMX2 antibody [EPR14394] (ab180940)

    Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling RBMX2 with ab180940 at a 1/100 dilution. Prediluted ImmunoHistoprobe (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMX2 antibody [EPR14394] (ab180940)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMX2 antibody [EPR14394] (ab180940)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling RBMX2 with ab180940 at a 1/100 dilution. Prediluted ImmunoHistoprobe (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-RBMX2 antibody [EPR14394] (ab180940)
    Immunocytochemistry/ Immunofluorescence - Anti-RBMX2 antibody [EPR14394] (ab180940)

    Immunofluorescent analysis of -20℃ acetone-fixed HeLa cell line staining RBMX2 using ab180940 at a 1/100 dilution. Goat anti rabbit IgG (Dylight 488), at a dilution of 1/200, was used as a secondary antibody. Counter stain: Dapi (blue).

  • Anti-RBMX2 antibody [EPR14394] (ab180940)
    Anti-RBMX2 antibody [EPR14394] (ab180940)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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