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Anti-p53 (phospho S20) antibody [EPR2156(2)] (ab157454)

Price and availability

324 988 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-p53 (phospho S20) antibody [EPR2156(2)] (ab157454)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2156(2)] to p53 (phospho S20)
  • Suitable for: WB, IP, Dot blot
  • Reacts with: Human

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Overview

  • Product name

    Anti-p53 (phospho S20) antibody [EPR2156(2)]
    See all p53 primary antibodies
  • Description

    Rabbit monoclonal [EPR2156(2)] to p53 (phospho S20)
  • Host species

    Rabbit
  • Specificity

    ab157454 only detects p53 phosphorylated at Serine 20.
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human p53 aa 1-100 (phospho S20). The exact sequence is proprietary.
    Database link: P04637

  • Positive control

    • T47D cell lysates, treated with etoposide.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR2156(2)
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • p53
    • Cell Biology
    • Apoptosis
    • Intracellular
    • p53 Pathway
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • p53
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Cycle Inhibitors
    • p53
    • Cancer
    • Cell cycle
    • Cell cycle inhibitors
    • p53 pathway
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • p53 pathway

Images

  • Western blot - Anti-p53 (phospho S20) antibody [EPR2156(2)] (ab157454)
    Western blot - Anti-p53 (phospho S20) antibody [EPR2156(2)] (ab157454)
    All lanes : Anti-p53 (phospho S20) antibody [EPR2156(2)] (ab157454) at 1/1000 dilution

    Lane 1 : T47D (human mammary gland ductal carcinoma) treated with Etoposide whole cell lysate
    Lane 2 : Untreated T47D (human mammary gland ductal carcinoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 53 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking & dilution buffer: 5% NFDM/TBST

  • Immunoprecipitation - Anti-p53 (phospho S20) antibody [EPR2156(2)] (ab157454)
    Immunoprecipitation - Anti-p53 (phospho S20) antibody [EPR2156(2)] (ab157454)
    Detection of p53 Western Blot of Immunprecipitate. T47D cells treated with etoposide lysate immunoprecipitated using ab157454 at 1/10 dilution followed by HRP-conjugated anti-rabbit IgG, which preferentially detects the non-reduced form of rabbit IgG.

    Anti-p53 (phospho S20) antibody [EPR2156(2)] (ab157454) at 1/1000 dilution + Immunoprecipitation pellet from T47-D cells treated with etoposide lysate at 10 µg
  • Western blot - Anti-p53 (phospho S20) antibody [EPR2156(2)] (ab157454)
    Western blot - Anti-p53 (phospho S20) antibody [EPR2156(2)] (ab157454)
    All lanes : Anti-p53 (phospho S20) antibody [EPR2156(2)] (ab157454) at 1/1000 dilution

    Lane 1 : T47D cell lysate
    Lane 2 : T47D cell lysate treated with etoposide.

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 44 kDa



    Lower panel shows labeling with an anti-p53 C terminal antibody.
  • Dot Blot - Anti-p53 (phospho S20) antibody [EPR2156(2)] (ab157454)
    Dot Blot - Anti-p53 (phospho S20) antibody [EPR2156(2)] (ab157454)

    Primary antibody dilution: 1/1000

    Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated 

    Secondary antibody dilution: 1/2500

    Blocking & dilution buffer: 5% NFDM/TBST

    Lane 1 sample: p53 (pS20) phospho peptide

    Lane 2 sample: p53 non-phospho peptide

    Exposure time: 10 seconds

     

  • Anti-p53 (phospho S20) antibody [EPR2156(2)] (ab157454)
    Anti-p53 (phospho S20) antibody [EPR2156(2)] (ab157454)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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