Anti-p53 (phospho S20) antibody [EPR2156(2)] (ab157454)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2156(2)] to p53 (phospho S20)
- Suitable for: WB, IP, Dot blot
- Reacts with: Human
Overview
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Product name
Anti-p53 (phospho S20) antibody [EPR2156(2)]
See all p53 primary antibodies -
Description
Rabbit monoclonal [EPR2156(2)] to p53 (phospho S20) -
Host species
Rabbit -
Specificity
ab157454 only detects p53 phosphorylated at Serine 20. -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human -
Immunogen
Synthetic peptide within Human p53 aa 1-100 (phospho S20). The exact sequence is proprietary.
Database link: P04637 -
Positive control
- T47D cell lysates, treated with etoposide.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2156(2) -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-p53 (phospho S20) antibody [EPR2156(2)] (ab157454) at 1/1000 dilution
Lane 1 : T47D (human mammary gland ductal carcinoma) treated with Etoposide whole cell lysate
Lane 2 : Untreated T47D (human mammary gland ductal carcinoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Predicted band size: 44 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking & dilution buffer: 5% NFDM/TBST
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Detection of p53 Western Blot of Immunprecipitate. T47D cells treated with etoposide lysate immunoprecipitated using ab157454 at 1/10 dilution followed by HRP-conjugated anti-rabbit IgG, which preferentially detects the non-reduced form of rabbit IgG.
Anti-p53 (phospho S20) antibody [EPR2156(2)] (ab157454) at 1/1000 dilution + Immunoprecipitation pellet from T47-D cells treated with etoposide lysate at 10 µg
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All lanes : Anti-p53 (phospho S20) antibody [EPR2156(2)] (ab157454) at 1/1000 dilution
Lane 1 : T47D cell lysate
Lane 2 : T47D cell lysate treated with etoposide.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 44 kDa
Lower panel shows labeling with an anti-p53 C terminal antibody. -
Primary antibody dilution: 1/1000
Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated
Secondary antibody dilution: 1/2500
Blocking & dilution buffer: 5% NFDM/TBST
Lane 1 sample: p53 (pS20) phospho peptide
Lane 2 sample: p53 non-phospho peptide
Exposure time: 10 seconds
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