Antibodies, Proteins, Kits and Reagents for Life Science

351 792 ₸ Product size

100 µl 351 792 ₸ 10 µl 140 716 ₸

Order now and get it on Tuesday March 02, 2021

Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4041(2)] to GRP78 BiP
Suitable for: Flow Cyt, WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-GRP78 BiP antibody [EPR4041(2)]
See all GRP78 BiP primary antibodies
Description

Rabbit monoclonal [EPR4041(2)] to GRP78 BiP
Host species

Rabbit
Specificity

Stimulation may be required to allow detection of the target protein due to low levels of endogenous expression in some samples. Please see images below for recommended treatment conditions and positive controls.

The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, NIH/3T3, LnCaP, MCF7, 293T, U-87 MG, THP-1, MDA-MB-231 and HepG2 whole cell lysate (ab7900); Human liver lysates, Mouse heart, lung, liver and testis lysates; Rat liver lysates. IHC-P: Human breast carcinoma Tissue Flow Cyt: HeLa cells
General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4041(2)
Isotype

IgG
Research areas

Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)

All lanes : Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/1000 dilution

Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lane 3 : 293T (Human embryonic kidney epithelial cell) whole cell lysates
Lane 4 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates
Lane 5 : THP-1 (Human monocytic leukemia monocyte) whole cell lysates
Lane 6 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 7 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
Lane 8 : Human uterus lysates
Lane 9 : Human liver lysates
Lane 10 : Mouse heart lysates
Lane 11 : Mouse lung lysates
Lane 12 : Mouse testis lysates
Lane 13 : Mouse liver lysates
Lane 14 : Rat liver lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 72 kDa
Observed band size: 78 kDa
why is the actual band size different from the predicted?

Exposure time: 26 seconds

Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)

Immunohistochemical staining of paraffin embedded human hepatocellular carcinoma with purified ab108615 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Flow Cytometry - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)

Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling GRP78 BiP with purified ab108615 at 1/200 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)

All lanes : Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/1000 dilution

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HeLa treated with 2.5 µg/ml tunicamycin for 24h whole cell lysates
Lane 3 : HUVEC (Human umbilical vein endothelial cell) whole cell lysates
Lane 4 : HUVEC treated with 10 µg/ml tunicamycin for 48h whole cell lysates
Lane 5 : Raw 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
Lane 6 : Raw 264.7 treated with 5 µg/ml tunicamycin for 18h whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 72 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?

Exposure time: 3 seconds
Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)

All lanes : Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/1000 dilution (purified)

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 10ug/ml
tunicamycin for 12 hours whole cell lysates
Lane 3 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 4 : MCF-7 (Human breast adenocarcinoma epithelial cell) treated with 10ug/ml
tunicamycin for 48 hours whole cell lysates
Lane 5 : MEF (Mouse embryonic fibroblast) whole cell lysates
Lane 6 : MEF (Mouse embryonic fibroblast) treated with 10ug/ml tunicamycin for 48 hours whole cell lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 72 kDa

Blocking and diluting buffer: 5% NFDM/TBST.
Tunicamycin induces the expression of GRP78 BiP reported by PMID 22859938

Exposure time:
Lanes 1 and 2: 20 seconds
Lanes 3 and 4: 30 seconds
Lanes 5 and 6: 5 seconds
Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)

All lanes : Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/1000 dilution (unpurified)

Lane 1 : HeLa cell lysates
Lane 2 : NIH/3T3 cell lysates
Lane 3 : LnCaP cell lysates
Lane 4 : HepG2 cell lysates

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 72 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)

Unpurified ab108615 at 1/50 dilution staining GRP78 BiP in Human breast carcinoma tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)

Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/1000 dilution (purified) + Raw264.7 cell lysate at 20 µg

Secondary
HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

Predicted band size: 72 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)

Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/5000 dilution (purified) + HepG2 at 20 µg

Secondary
HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

Predicted band size: 72 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)

Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/1000 dilution (purified) + LnCaP at 20 µg

Secondary
HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

Predicted band size: 72 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)

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