Anti-GRP78 BiP antibody (ab230508)
Key features and details
- Rabbit polyclonal to GRP78 BiP
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-GRP78 BiP antibody
See all GRP78 BiP primary antibodies -
Description
Rabbit polyclonal to GRP78 BiP -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Monkey
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Immunogen
Synthetic peptide within Human GRP78 BiP (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P11021 -
Positive control
- IHC-P: Human kidney and lung tissues. ICC/IF: HeLa cells. WB: HepG2, HeLa, Raw264.7 and mouse brain whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GRP78 BiP antibody (ab230508)All lanes : Anti-GRP78 BiP antibody (ab230508) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : Raw264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 4 : Mouse brain whole cell lysate
Predicted band size: 72 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-GRP78 BiP antibody (ab230508)Formalin-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GRP78 BiP (red) using ab230508 at 1/100 dilution and icubated with DyLight 594-conjugated secondary antibody PBS at room temperature in the dark.
Nucelar counterstain: DAPI (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody (ab230508)Formalin-fixed, paraffin-embedded human lung tissue stained for GRP78 BiP using ab230508 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody (ab230508)Formalin-fixed, paraffin-embedded human kidney tissue stained for GRP78 BiP using ab230508 at 1/100 dilution in immunohistochemical analysis.
