Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸ 10 µl 113 913 ₸

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Anti-GRP78 BiP antibody [EPR21226] (ab213258)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21226] to GRP78 BiP
Suitable for: ICC/IF
Reacts with: Mouse, Human

Product name

Anti-GRP78 BiP antibody [EPR21226]
See all GRP78 BiP primary antibodies
Description

Rabbit monoclonal [EPR21226] to GRP78 BiP
Host species

Rabbit
Tested Applications & Species

Application Species
ICC/IF
Mouse

Human

See all applications and species data
Immunogen

Recombinant fragment.
Database link: P11021
Positive control
- ICC-IF: HeLa and NIH3T3 cell lines.
General notes

This product was made using synthetic libraries and phage display technology.

This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
ICC/IF	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21226
Isotype

IgG1
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-GRP78 BiP antibody [EPR21226] (ab213258)

ab213258 staining GRP78 BiP in HeLa cells. The cells were treated with 5ug/ml Brefeldin A for 30 min and then fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab213258 at 0.2ugml then detected with an Alexa Fluor^® 488 goat anti-rabbit secondary antibody (ab150081) at a 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue), and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at a 1/250 dilution (shown in red).
Immunocytochemistry/ Immunofluorescence - Anti-GRP78 BiP antibody [EPR21226] (ab213258)

ab213258 staining GRP78 BiP in NIH3T3 cells. The cells were treated with 5ug/ml Brefeldin A for 30 min and then fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab213258 at 0.2μg/ml then detected with an Alexa Fluor^® 488 goat anti-rabbit secondary antibody (ab150081) at a 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue), and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at a 1/250 dilution (shown in red).
Anti-GRP78 BiP antibody [EPR21226] (ab213258)

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