APC Anti-CD47 antibody [OX-101] (ab256293)
![APC Anti-CD47 antibody [OX-101] (ab256293)](https://www.abcam.com/ps/products/256/ab256293/Images/ab256293-1-ab256293antiratCD47flowcytometryspleensplenocytes.jpg "APC Anti-CD47 antibody [OX-101] (ab256293)")
Key features and details
- APC Mouse monoclonal [OX-101] to CD47
- Suitable for: Flow Cyt
- Reacts with: Rat
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG1
Overview
-
Product name
APC Anti-CD47 antibody [OX-101]
See all CD47 primary antibodies -
Description
APC Mouse monoclonal [OX-101] to CD47 -
Host species
Mouse -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Rat
-
Immunogen
Tissue, cells or virus corresponding to Rat CD47. Biotinylated rat (AO) thymocytes.
-
Positive control
- Flow Cyt: Lewis rat splenocytes.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Store In the Dark. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
OX-101 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
-
Flow Cytometry - APC Anti-CD47 antibody [OX-101] (ab256293)
Lewis rat splenocytes stained with ab256293 (right) or mouse IgG1κ Allophycocyanin isotype (left). Lewis rat splenocytes were incubated for 30 min on ice in 1x PBS / 10 % rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab256293) or mouse IgG1κ Allophycocyanin isotype (1x 106 in 100 µl at 0.2 µg/ml (1/2500)) for 30 min on ice. The cells were simultaneously stained with CD3.
Acquisition of >30,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter. Events were gated on viable lymphocytes.
