Anti-RABL2A antibody [OTI5A10] (ab119412)
Key features and details
- Mouse monoclonal [OTI5A10] to RABL2A
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-RABL2A antibody [OTI5A10] -
Description
Mouse monoclonal [OTI5A10] to RABL2A -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human RABL2A aa 1-250. Produced in HEK-293T cells (NP_009013).
Database link: Q9UBK7 -
Positive control
- WB: HEK-293T cells transfected with pCMV6-ENTRY RABL2A cDNA. IHC-P: Human breast, endometrium, prostate, bladder carcinoma, ovary adenocarcinoma and endometrium adenocarcinoma tissue.
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General notes
Clone OTI5A10 (formerly 5A10).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant by affinity chromatography. -
Clonality
Monoclonal -
Clone number
OTI5A10 -
Isotype
IgG1 -
Research areas
Images
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All lanes : Anti-RABL2A antibody [OTI5A10] (ab119412) (1/2000)
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY RABL2A cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 26 kDa
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Paraffin-embedded human breast tissue stained for RABL2A with ab119412 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
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Paraffin-embedded human ovary adenocarcinoma tissue stained for RABL2A with ab119412 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
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Paraffin-embedded human endometrium tissue stained for RABL2A with ab119412 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
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Paraffin-embedded human endometrium adenocarcinoma tissue stained for RABL2A with ab119412 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
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Paraffin-embedded human prostate tissue stained for RABL2A with ab119412 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
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Paraffin-embedded human bladder carcinoma tissue stained for RABL2A with ab119412 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.