Antibodies, Proteins, Kits and Reagents for Life Science

Anti-RABL2A antibody [OTI5A10] (ab119412)

Key features and details

Mouse monoclonal [OTI5A10] to RABL2A
Suitable for: WB, IHC-P
Reacts with: Human
Isotype: IgG1

Product name

Anti-RABL2A antibody [OTI5A10]
Description

Mouse monoclonal [OTI5A10] to RABL2A
Host species

Mouse
Tested applications

Suitable for: WB, IHC-Pmore details
Species reactivity

Reacts with: Human
Immunogen

Recombinant full length protein corresponding to Human RABL2A aa 1-250. Produced in HEK-293T cells (NP_009013).
Database link: Q9UBK7

Run BLAST with Run BLAST with
Positive control
- WB: HEK-293T cells transfected with pCMV6-ENTRY RABL2A cDNA. IHC-P: Human breast, endometrium, prostate, bladder carcinoma, ovary adenocarcinoma and endometrium adenocarcinoma tissue.
General notes

Clone OTI5A10 (formerly 5A10).

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA
Concentration information loading...
Purity

Affinity purified
Purification notes

Purified from cell culture supernatant by affinity chromatography.
Clonality

Monoclonal
Clone number

OTI5A10
Isotype

IgG1
Research areas

Western blot - Anti-RABL2A antibody [OTI5A10] (ab119412)

All lanes : Anti-RABL2A antibody [OTI5A10] (ab119412) (1/2000)

Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY RABL2A cDNA

Lysates/proteins at 5 µg per lane.

Predicted band size: 26 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RABL2A antibody [OTI5A10] (ab119412)

Paraffin-embedded human breast tissue stained for RABL2A with ab119412 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RABL2A antibody [OTI5A10] (ab119412)

Paraffin-embedded human ovary adenocarcinoma tissue stained for RABL2A with ab119412 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RABL2A antibody [OTI5A10] (ab119412)

Paraffin-embedded human endometrium tissue stained for RABL2A with ab119412 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RABL2A antibody [OTI5A10] (ab119412)

Paraffin-embedded human endometrium adenocarcinoma tissue stained for RABL2A with ab119412 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RABL2A antibody [OTI5A10] (ab119412)

Paraffin-embedded human prostate tissue stained for RABL2A with ab119412 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RABL2A antibody [OTI5A10] (ab119412)

Paraffin-embedded human bladder carcinoma tissue stained for RABL2A with ab119412 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

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