Anti-ARF1+ARF3 antibody [EP442Y] - BSA and Azide free (ab247266)
![Anti-ARF1+ARF3 antibody [EP442Y] - BSA and Azide free (ab247266)](https://www.abcam.com/ps/products/247/ab247266/Images/ab247266-410500-anti-arf1-arf3-antibody-ep442y-bsa-and-azide-free-western-blot.jpg "Anti-ARF1+ARF3 antibody [EP442Y] - BSA and Azide free (ab247266)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP442Y] to ARF1+ARF3 - BSA and Azide free
- Suitable for: IHC-P, ICC, WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-ARF1+ARF3 antibody [EP442Y] - BSA and Azide free
See all ARF1+ARF3 primary antibodies -
Description
Rabbit monoclonal [EP442Y] to ARF1+ARF3 - BSA and Azide free -
Host species
Rabbit -
Specificity
The immunogen used for this product shares 100% homology with ARF3, ARF4 and ARF5. Cross-reactivity with the latter two protein have not been confirmed experimentally.
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Tested applications
Suitable for: IHC-P, ICC, WB, Flow Cytmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab247266 is the carrier-free version of ab32524. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab247266 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
EP442Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ARF1+ARF3 antibody [EP442Y] - BSA and Azide free (ab247266)All lanes : Anti-ARF1 + ARF3 antibody [EP442Y] (ab32524) at 1/20000 dilution
Lane 1 : Full length human ARF-1 recombinant protein
Lane 2 : Full length human ARF-3 recombinant protein
Lysates/proteins at 0.015 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab32524, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Human ARF-1 recombinant protein (ab123538 ), Human ARF-3 recombinant protein (ab113581).
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![Immunocytochemistry - Anti-ARF1+ARF3 antibody [EP442Y] - BSA and Azide free (ab247266)](https://www.abcam.com/ps/products/247/ab247266/Images/ab247266-4-anti-arf1-arf3-antibody-ep442y-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-ARF1+ARF3 antibody [EP442Y] - BSA and Azide free (ab247266)This data was developed using ab32524, the same antibody clone in a different buffer formulation.Immunofluorescence staining of MCF7 cells with purified ab32524 working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 100% methanol. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody. -
![Western blot - Anti-ARF1+ARF3 antibody [EP442Y] - BSA and Azide free (ab247266)](https://www.abcam.com/ps/products/247/ab247266/Images/ab247266-405428-anti-arf1-arf3-antibody-ep442y-bsa-and-azide-free-western-blot.gif)
Western blot - Anti-ARF1+ARF3 antibody [EP442Y] - BSA and Azide free (ab247266)Anti-ARF1 + ARF3 antibody [EP442Y] (ab32524) at 1/2000 dilution + HEK-293 cell lysate
Predicted band size: 21 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?This data was developed using ab32524, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1+ARF3 antibody [EP442Y] - BSA and Azide free (ab247266)](https://www.abcam.com/ps/products/247/ab247266/Images/ab247266-2-anti-arf1-arf3-antibody-ep442y-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1+ARF3 antibody [EP442Y] - BSA and Azide free (ab247266)This data was developed using ab32524, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab32524 at 1/250 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Flow Cytometry - Anti-ARF1+ARF3 antibody [EP442Y] - BSA and Azide free (ab247266)](https://www.abcam.com/ps/products/247/ab247266/Images/ab247266-1-anti-arf1-arf3-antibody-ep442y-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-ARF1+ARF3 antibody [EP442Y] - BSA and Azide free (ab247266)This data was developed using ab32524, the same antibody clone in a different buffer formulation.Overlay histogram showing HeLa cells stained with ab32524 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32524, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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![Anti-ARF1+ARF3 antibody [EP442Y] - BSA and Azide free (ab247266)](https://www.abcam.com/ps/products/247/ab247266/Images/ab247266-6-benefits-of-recombinant-antibodies.png)
Anti-ARF1+ARF3 antibody [EP442Y] - BSA and Azide free (ab247266)
