HRP Anti-RAB8A antibody [EPR14873] - C-terminal (ab209046)
![HRP Anti-RAB8A antibody [EPR14873] - C-terminal (ab209046)](https://www.abcam.com/ps/products/209/ab209046/Images/ab209046-270994-anti-rab8a-antibody-epr14873-c-terminal-hrp-immunohistochemistry.jpg "HRP Anti-RAB8A antibody [EPR14873] - C-terminal (ab209046)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR14873] to RAB8A - C-terminal
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Rat, Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-RAB8A antibody [EPR14873] - C-terminal
See all RAB8A primary antibodies -
Description
HRP Rabbit monoclonal [EPR14873] to RAB8A - C-terminal -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, HCT 116, PC12 and wild-type HAP1 whole cell lysates. IHC-P: Normal human colon tissue sections.
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General notes
Alternative versions available:
Anti-RAB8A antibody [EPR14873] - C-terminal (ab188574) - Knockout validatedThis product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14873 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-RAB8A antibody [EPR14873] - C-terminal (ab209046)
IHC image of RAB8A staining in a section of formalin-fixed paraffin-embedded normal human colon*, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab209046, 1/50 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Western blot - HRP Anti-RAB8A antibody [EPR14873] - C-terminal (ab209046)](https://www.abcam.com/ps/products/209/ab209046/Images/ab209046-265730-anti-rab8a-antibody-epr14873-c-terminal-hrp-western-blot.jpg)
Western blot - HRP Anti-RAB8A antibody [EPR14873] - C-terminal (ab209046)All lanes : HRP Anti-RAB8A antibody [EPR14873] - C-terminal (ab209046) at 1/5000 dilution
Lane 1 :HeLa whole cell lysate (ab150035) at 10 µg
Lane 2 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate at 10 µg
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
Lane 4 : Wild-type HAP1 cell lysate at 20 µg
Lane 5 : RAB8A knockout HAP1 cell lysate at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 24 kDa
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab209046 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-RAB8A antibody [EPR14873] - C-terminal (ab209046)](https://www.abcam.com/ps/products/209/ab209046/Images/ab209046-3-benefits-of-recombinant-antibodies.png)
HRP Anti-RAB8A antibody [EPR14873] - C-terminal (ab209046)
