Antibodies, Proteins, Kits and Reagents for Life Science

154 118 ₸ Product size

100 µl 154 118 ₸

Order now and get it on Wednesday March 03, 2021

Anti-RAB8A antibody [MJF-R22] (ab237702)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [MJF-R22] to RAB8A
Suitable for: WB, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-RAB8A antibody [MJF-R22]
See all RAB8A primary antibodies
Description

Rabbit monoclonal [MJF-R22] to RAB8A
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Mouse

Human

WB
Human

See all applications and species data
Immunogen

Recombinant full length protein corresponding to Human RAB8A aa 1 to the C-terminus. Antibody epitope is C-terminal amino acids KITPDQQ (AA190-196)
Database link: P61006

Run BLAST with Run BLAST with
Positive control
- WB: A549, MCF7, NIH/3T3, PC-12 and C6 whole cell lysates. IP: A549, NIH/3T3 and MEF whole cell lysates.
General notes
This antibody was developed with support from The Michael J. Fox Foundation.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Mouse Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

MJF-R22
Isotype

IgG
Research areas

Immunoprecipitation - Anti-RAB8A antibody [MJF-R22] (ab237702)

RAB8A was immunoprecipitated from varying amounts of MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate with 1 μg of ab237702 per IP test. Western blot was perfromed from the immunoprecipitate using ab237702 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/2000 dilution.

Lane 1: MEF whole cell lysate (input).
Lane 2-7: ab237702 IP in different amounts of MEF lysates from 25 µg to 800 µg whole cell lysate.
Lane 8: Pre-immune IgG instead of ab237702 in MEF whole cell lysate.

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting Buffer and concentration: 5% BSA/TBST.

Exposure time: 10 seconds.

This image is kindly provided by our collaborator Dr. Dario Alessi, University of Dundee.
Immunoprecipitation - Anti-RAB8A antibody [MJF-R22] (ab237702)

RAB8A was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate using ab237702 at 1/30. Western blot was performed from the immunoprecipitate using ab237702 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

Lane 1: NIH/3T3 whole cell lysate 10μg (Input).
Lane 2: ab237702 IP in NIH/3T3 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab237702 in NIH/3T3 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
Immunoprecipitation - Anti-RAB8A antibody [MJF-R22] (ab237702)

RAB8A was immunoprecipitated from 0.35 mg A549 (human lung carcinoma epithelial cell line) whole cell lysate using ab237702 at 1/30. Western blot was performed from the immunoprecipitate using ab237702 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

Lane 1: A549 (Human lung carcinoma epithelial cell) whole cell lysate 10μg (Input).
Lane 2: ab237702 IP in A459 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab237702 in A549 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
Western blot - Anti-RAB8A antibody [MJF-R22] (ab237702)

All lanes : Anti-RAB8A antibody [MJF-R22] (ab237702) at 1/1000 dilution

Lane 1 : Wild-type A549 (human lung carcinoma epithelial cell) whole cell lysate
Lane 2 : Rab8A knockout A549 whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 5 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
Lane 6 : C6 (rat glial tumor glial cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 24 kDa
Observed band size: 24 kDa

Exposure time: 10 seconds

Blocking/Diluting buffer and concentration: 5% NFDM/TBST

The band around 50 kDa is non-specific band.

The WT and Rab8A KO A549 lysates were kindly provided by our collaborator Dr. Dario Alessi, University of Dundee.
Anti-RAB8A antibody [MJF-R22] (ab237702)

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