Anti-CCR2 antibody [EPR19698] - Low endotoxin, Azide free (ab222496)
![Anti-CCR2 antibody [EPR19698] - Low endotoxin, Azide free (ab222496)](https://www.abcam.com/ps/products/222/ab222496/Images/ab222496-320900-anti-ccr2-antibody-epr19698-immunofluorescence.jpg "Anti-CCR2 antibody [EPR19698] - Low endotoxin, Azide free (ab222496)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19698] to CCR2 - Low endotoxin, Azide free
- Suitable for: WB, ICC/IF
- Reacts with: Mouse
Overview
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Product name
Anti-CCR2 antibody [EPR19698] - Low endotoxin, Azide free
See all CCR2 primary antibodies -
Description
Rabbit monoclonal [EPR19698] to CCR2 - Low endotoxin, Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Mouse
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Immunogen
This product was produced with the following immunogens:
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Recombinant fragment within Mouse CCR2 aa 300 to the C-terminus. The exact sequence is proprietary.
Database link: P51683 -
Positive control
- WB: Mouse spleen and brain lysates; RAW 264.7 and NIH/3T3 whole cell lysates; J774A.1 whole cell lysates untreated and treated with 500U/ml IFN gamma for 4 hours. ICC/IF: RAW 264.7 and J774A.1 cells.
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General notes
ab222496 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19698 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR19698] - Low endotoxin, Azide free (ab222496)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling CCR2 with ab203128 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic staining on RAW 264.7 cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203128).
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![Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR19698] - Low endotoxin, Azide free (ab222496)](https://www.abcam.com/ps/products/222/ab222496/Images/ab222496-320899-anti-ccr2-antibody-epr19698-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR19698] - Low endotoxin, Azide free (ab222496)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized J774A.1 (Mouse macrophage reticulum cell sarcoma cell line) cells labeling CCR2 with ab203128 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic staining on J774A.1 cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203128).
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![Anti-CCR2 antibody [EPR19698] - Low endotoxin, Azide free (ab222496)](https://www.abcam.com/ps/products/222/ab222496/Images/ab222496-3-benefits-of-recombinant-antibodies.png)
Anti-CCR2 antibody [EPR19698] - Low endotoxin, Azide free (ab222496)
