Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free
See all Annexin A1/ANXA1 primary antibodies
Description

Mouse monoclonal [ANXA1/3566] to Annexin A1/ANXA1 - BSA and Azide free
Host species

Mouse
Tested applications

Suitable for: Flow Cyt, ICC/IF, IHC-P, Protein Array, WBmore details
Species reactivity

Reacts with: Human
Immunogen

Recombinant full length protein within Human Annexin A1/ANXA1 aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P04083
Positive control
- WB: A549 cell lysate. IHC-P: Human lymph node tissue. Human colon carcinoma and prostate carcinoma tissue. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
General notes

ab268191 is the carrier-free version of ab268070.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from bioreactor concentrate.
Clonality

Monoclonal
Clone number

ANXA1/3566
Isotype

IgG2a
Light chain type

kappa
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin I antibody [ANXA1/3566] - BSA and Azide free (ab268191)

Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Annexin I using ab268070 at 2 μg/ml in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268070)
Immunocytochemistry/ Immunofluorescence - Anti-Annexin I antibody [ANXA1/3566] - BSA and Azide free (ab268191)

Immunofluorescence staining of paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Annexin I with ab268070 at 2 µg/ml followed by a goat anti-Mouse IgG-CF488 (Green). Nuclei are labeled with Reddot (Red).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268070)
Western blot - Anti-Annexin I antibody [ANXA1/3566] - BSA and Azide free (ab268191)

Anti-Annexin I antibody [ANXA1/3566] (ab268070) at 2 µg/ml + A549 (Human lung carcinoma cell line) whole cell lysate

Predicted band size: 39 kDa

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268070)
Flow Cytometry - Anti-Annexin I antibody [ANXA1/3566] - BSA and Azide free (ab268191)

Flow cytometric analysis of PFA-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Annexin I using ab268070 at 2 μg/million cells followed by a goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268070)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin I antibody [ANXA1/3566] - BSA and Azide free (ab268191)

Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for Annexin I using ab268070 at 2 μg/ml in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268070)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin I antibody [ANXA1/3566] - BSA and Azide free (ab268191)

Formalin-fixed, paraffin-embedded human lymph node tissue stained for Annexin I using ab268070 at 2 μg/ml in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268070)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin I antibody [ANXA1/3566] - BSA and Azide free (ab268191)

Formalin-fixed, paraffin-embedded human lymph node tissue stained for Annexin I using ab268070 at 2 μg/ml in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268070)
Protein Array - Anti-Annexin I antibody [ANXA1/3566] - BSA and Azide free (ab268191)

Protein Array containing more than 19,000 full-length human proteins using ab268070.

Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268070)