Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Annexin-2/ANXA2 antibody [D1/274.5] - BSA and Azide free (ab269512)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [D1/274.5] to Annexin-2/ANXA2 - BSA and Azide free
  • Suitable for: ICC/IF, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Annexin-2/ANXA2 antibody [D1/274.5] - BSA and Azide free
    See all Annexin-2/ANXA2 primary antibodies

  • Description

    Mouse monoclonal [D1/274.5] to Annexin-2/ANXA2 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC/IF, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Tissue, cells or virus within Human Annexin-2/ANXA2. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Human ovary cancer tissue lysate. HeLa and HUVEC whole cell lysate. ICC/IF: HeLa and MCF7 cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab269512 is the carrier-free version of ab269511. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-Annexin-2/ANXA2 antibody [D1/274.5] - BSA and Azide free (ab269512)
    Western blot - Anti-Annexin-2/ANXA2 antibody [D1/274.5] - BSA and Azide free (ab269512)
    All lanes : Anti-Annexin-2/ANXA2 antibody [D1/274.5] (ab269511) at 0.773 µg/ml

    Lane 1 : Human ovary cancer tissue lysate
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 3 : HUVEC (human umbilical vein endothelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Predicted band size: 38 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Lane 2-3 of this blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269511).

  • Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody [D1/274.5] - BSA and Azide free (ab269512)
    Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody [D1/274.5] - BSA and Azide free (ab269512)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MCF7 9human breast adenocarcinoma cell line) cells labelling Annexin II with ab269511 at 15.46 µg/mL, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing strongly membranous and cytoplasmic staining in MCF7 cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269511).

  • Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody [D1/274.5] - BSA and Azide free (ab269512)
    Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody [D1/274.5] - BSA and Azide free (ab269512)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling Annexin II with ab269511 at 15.46 µg/mL, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing strongly membranous and cytoplasmic staining in HeLa cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269511).

  • Anti-Annexin-2/ANXA2 antibody [D1/274.5] - BSA and Azide free (ab269512)
    Anti-Annexin-2/ANXA2 antibody [D1/274.5] - BSA and Azide free (ab269512)

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