Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] - BSA and Azide free (ab256535)
![Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] - BSA and Azide free (ab256535)](https://www.abcam.com/ps/products/256/ab256535/Images/ab256535-4-IP1anti-kir2dl2-kir2dl1-kir2dl3-antibody-epr22541-47-immunoprecipitation-nk92-human.jpg "Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] - BSA and Azide free (ab256535)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22541-47] to KIR2DL3 + KIR2DL1 + KIR2DL2 - BSA and Azide free
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
- Reacts with: Human
Overview
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Product name
Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] - BSA and Azide free -
Description
Rabbit monoclonal [EPR22541-47] to KIR2DL3 + KIR2DL1 + KIR2DL2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human endometrium tissue. ICC/IF: NK-92 cells treated with 5-aza-2-deoxycytidine (2 uM) for 48 h. Flow Cyt: Human PBMCs. IP: NK-92 cells treated with 5-aza-2-deoxycytidine (2 uM) for 48 h, whole cell lysate.
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General notes
ab256535 is the carrier-free version of ab224697 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab256535 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22541-47 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] - BSA and Azide free (ab256535)
KIR2DL2+KIR2DL1+KIR2DL3 was immunoprecipitated from 0.35 mg NK-92 (Human malignant non-Hodgkin's lymphoma natural killer cell) treated with 2uM 5-aza-2-deoxycytidine for 48h whole cell lysate 10µg with ab224697 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab224697. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: NK-92 (Human malignant non-Hodgkin's lymphoma natural killer cell) treated with 2µM 5-aza-2-deoxycytidine for 48h whole cell lysate 10µg.
Lane 2: ab224697 IP in NK-92 treated with 2µM 5-aza-2-deoxycytidine for 48h whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab224697 in NK-92 treated with 2µM 5-aza-2-deoxycytidine for 48h whole cell lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224697).
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![Flow Cytometry - Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] - BSA and Azide free (ab256535)](https://www.abcam.com/ps/products/256/ab256535/Images/ab256535-3-FC1anti-kir2dl2-kir2dl1-kir2dl3-antibody-epr22541-47-flowcytometry-pbmc-human.jpg)
Flow Cytometry - Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] - BSA and Azide free (ab256535)Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling KIR2DL2+KIR2DL1+KIR2DL3 with ab224697 at 1/600 compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. Goat anti rabbit IgG (Alexa Fluor® 488, ab150097) at 1/5000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG (Left) or ab224697 (Right). Then stained with anti-CD56 conjugated to BV421. Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224697).
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![Immunocytochemistry/ Immunofluorescence - Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] - BSA and Azide free (ab256535)](https://www.abcam.com/ps/products/256/ab256535/Images/ab256535-2-ICC1anti-kir2dl2-kir2dl1-kir2dl3-antibody-epr22541-47-immunocytochemistry-nk92-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] - BSA and Azide free (ab256535)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NK-92 (human malignant non-Hodgkin's lymphoma natural killer cell) cells labeling KIR2DL2+KIR2DL1+KIR2DL3 with ab224697 at 1/100, 5.7µg/ml dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000, 2µg/ml dilution (Green). Confocal image showing membranous and cytoplasmic staining in NK-92 cells treated with 5-aza-2-deoxycytidine (2µM) for 48 h is observed. ab195889 anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control/ Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000, 2µg/ml dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224697).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] - BSA and Azide free (ab256535)](https://www.abcam.com/ps/products/256/ab256535/Images/ab256535-1-IHC1anti-kir2dl2-kir2dl1-kir2dl3-antibody-epr22541-47-immunohistochemistry-endometrium-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] - BSA and Azide free (ab256535)Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling KIR2DL2+KIR2DL1+KIR2DL3 with ab224697 at 1/500 dilution (1.13µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in natural killer (NK) cells in human endometrium (PMID: 7749980). Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224697).
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![Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] - BSA and Azide free (ab256535)](https://www.abcam.com/ps/products/256/ab256535/Images/ab256535-5-benefits-of-recombinant-antibodies.png)
Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] - BSA and Azide free (ab256535)
