Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] - BSA and Azide free (ab256535)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22541-47] to KIR2DL3 + KIR2DL1 + KIR2DL2 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] - BSA and Azide free

  • Description

    Rabbit monoclonal [EPR22541-47] to KIR2DL3 + KIR2DL1 + KIR2DL2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human endometrium tissue. ICC/IF: NK-92 cells treated with 5-aza-2-deoxycytidine (2 uM) for 48 h. Flow Cyt: Human PBMCs. IP: NK-92 cells treated with 5-aza-2-deoxycytidine (2 uM) for 48 h, whole cell lysate.

  • General notes

    ab256535 is the carrier-free version of ab224697 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab256535 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] - BSA and Azide free (ab256535)
    Immunoprecipitation - Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] - BSA and Azide free (ab256535)

    KIR2DL2+KIR2DL1+KIR2DL3 was immunoprecipitated from 0.35 mg NK-92 (Human malignant non-Hodgkin's lymphoma natural killer cell) treated with 2uM 5-aza-2-deoxycytidine for 48h whole cell lysate 10µg with ab224697 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab224697. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: NK-92 (Human malignant non-Hodgkin's lymphoma natural killer cell) treated with 2µM 5-aza-2-deoxycytidine for 48h whole cell lysate 10µg.

    Lane 2: ab224697 IP in NK-92 treated with 2µM 5-aza-2-deoxycytidine for 48h whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab224697 in NK-92 treated with 2µM 5-aza-2-deoxycytidine for 48h whole cell lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224697).

  • Flow Cytometry - Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] - BSA and Azide free (ab256535)
    Flow Cytometry - Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] - BSA and Azide free (ab256535)

    Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling KIR2DL2+KIR2DL1+KIR2DL3 with ab224697 at 1/600 compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. Goat anti rabbit IgG (Alexa Fluor® 488, ab150097) at 1/5000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG (Left) or ab224697 (Right). Then stained with anti-CD56 conjugated to BV421. Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224697).

  • Immunocytochemistry/ Immunofluorescence - Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] - BSA and Azide free (ab256535)
    Immunocytochemistry/ Immunofluorescence - Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] - BSA and Azide free (ab256535)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NK-92 (human malignant non-Hodgkin's lymphoma natural killer cell) cells labeling KIR2DL2+KIR2DL1+KIR2DL3 with ab224697 at 1/100, 5.7µg/ml dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000, 2µg/ml dilution (Green). Confocal image showing membranous and cytoplasmic staining in NK-92 cells treated with 5-aza-2-deoxycytidine (2µM) for 48 h is observed. ab195889 anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control/ Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000, 2µg/ml dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224697).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] - BSA and Azide free (ab256535)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] - BSA and Azide free (ab256535)

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling KIR2DL2+KIR2DL1+KIR2DL3 with ab224697 at 1/500 dilution (1.13µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in natural killer (NK) cells in human endometrium (PMID: 7749980). Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224697).

  • Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] - BSA and Azide free (ab256535)
    Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] - BSA and Azide free (ab256535)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.