Human SLAMF6 ELISA Kit (ab277424)
Key features and details
- Sensitivity: 0.12 ng/ml
- Range: 0.102 ng/ml - 25 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human SLAMF6 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.12 ng/ml -
Range
0.102 ng/ml - 25 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 105.7 69% - 141% Plasma 95.62 70% - 143% Cell culture media 114.6 100% - 141% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human SLAMF6 ELISA kit (ab277424) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human SLAMF6 in serum (human SLAMF6 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Human SLAMF6 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human SLAMF6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human SLAMF6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human SLAMF6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent 1 x 15ml Biotinylated Anti-Human SLAMF6 Antibody 2 vials Anti-Human SLAMF6 coated Microplate 1 unit Human SLAMF6 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Triggers cytolytic activity only in natural killer cells (NK) expressing high surface densities of natural cytotoxicity receptors. -
Tissue specificity
Expressed by all (resting and activated) natural killer cells (NK), T- and B-lymphocytes. -
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Alternative names
- Activating NK receptor
- CD352
- KALI
see all -
Database links
- Entrez Gene: 114836 Human
- Omim: 606446 Human
- SwissProt: Q96DU3 Human
- Unigene: 492348 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.