Anti-Biglycan antibody (ab231297)
Key features and details
- Rabbit polyclonal to Biglycan
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-Biglycan antibody
See all Biglycan primary antibodies -
Description
Rabbit polyclonal to Biglycan -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human, Pig
Predicted to work with: Mouse, Rat, Sheep, Horse, Cow, Dog
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Immunogen
Recombinant fragment (His-tag) corresponding to Human Biglycan aa 200 to the C-terminus. Expressed in E.coli. N-terminal tag.
Database link: P21810 -
Positive control
- IHC-P: Human breast cancer, liver, prostate, cerebrum and kidney tissues. WB: Pig kidney and spleen lysates; Recombinant human Biglycan protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab231297 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biglycan antibody (ab231297)
Paraffin-embedded human breast cancer tissue stained for Biglycan using ab231297 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Western blot - Anti-Biglycan antibody (ab231297)All lanes : Anti-Biglycan antibody (ab231297) at 5 µg/ml
Lane 1 : Pig spleen lysate
Lane 2 : Pig kidney lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit antibody at 1/1000 dilution
Predicted band size: 42 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biglycan antibody (ab231297)Formalin-fixed, paraffin-embedded human liver tissue stained for Biglycan using ab231297 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biglycan antibody (ab231297)Paraffin-embedded human prostate tissue stained for Biglycan using ab231297 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biglycan antibody (ab231297)Paraffin-embedded human cerebrum tissue stained for Biglycan using ab231297 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biglycan antibody (ab231297)Paraffin-embedded human kidney tissue stained for Biglycan using ab231297 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Western blot - Anti-Biglycan antibody (ab231297)Anti-Biglycan antibody (ab231297) at 2 µg/ml + Pig kidney lysate
Predicted band size: 42 kDa
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Western blot - Anti-Biglycan antibody (ab231297)Anti-Biglycan antibody (ab231297) at 2 µg/ml + Pig spleen lysate
Predicted band size: 42 kDa
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Western blot - Anti-Biglycan antibody (ab231297)Anti-Biglycan antibody (ab231297) at 2 µg/ml + Recombinant human Biglycan protein
Predicted band size: 42 kDa
