Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Biglycan antibody [EPR20235] (ab209234)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20235] to Biglycan
  • Suitable for: IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Biglycan antibody [EPR20235]
    See all Biglycan primary antibodies

  • Description

    Rabbit monoclonal [EPR20235] to Biglycan

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human testis, kidney, bladder cancer and thyroid cancer tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biglycan antibody [EPR20235] (ab209234)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biglycan antibody [EPR20235] (ab209234)

    Immunohistochemical analysis of paraffin-embedded human testis tissue labeling Biglycan with ab209234 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Specific extracellular matrix staining on peritubular myoid cells and leydig cells of human testis is observed [PMID: 7626709]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biglycan antibody [EPR20235] (ab209234)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biglycan antibody [EPR20235] (ab209234)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Biglycan with ab209234 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on basement membrane and blood vessel of human kidney is observed [PMID: 11012890]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biglycan antibody [EPR20235] (ab209234)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biglycan antibody [EPR20235] (ab209234)

    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling Biglycan with ab209234 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on stromal cells of human bladder cancer is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biglycan antibody [EPR20235] (ab209234)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biglycan antibody [EPR20235] (ab209234)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue labeling Biglycan with ab209234 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on stromal cells of human thyroid cancer is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Biglycan antibody [EPR20235] (ab209234)
    Anti-Biglycan antibody [EPR20235] (ab209234)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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