Anti-ENPP1/PC1 antibody [EPR22262-22] (ab223268)
![Anti-ENPP1/PC1 antibody [EPR22262-22] (ab223268)](https://www.abcam.com/ps/products/223/ab223268/Images/ab223268-319496-anti-enpp1-antibody-epr22262-22-western-blot.jpg "Anti-ENPP1/PC1 antibody [EPR22262-22] (ab223268)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22262-22] to ENPP1/PC1
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
- Reacts with: Human
Overview
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Product name
Anti-ENPP1/PC1 antibody [EPR22262-22]
See all ENPP1/PC1 primary antibodies -
Description
Rabbit monoclonal [EPR22262-22] to ENPP1/PC1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanIP HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human kidney tissue lysate; MDA-MB-231, HepG2 and Huh7 whole cell lysate. IHC-P: Human pancreas and liver tissue. ICC/IF: HepG2 and MDA-MB-231 cells. Flow: HepG2 cells. IP: MDA-MB-231 whole cell lysate.
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General notes
This product was previously labelled as ENPP1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22262-22 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ENPP1/PC1 antibody [EPR22262-22] (ab223268)All lanes : Anti-ENPP1/PC1 antibody [EPR22262-22] (ab223268) at 1/1000 dilution
Lane 1 : Human kidney tissue lysate
Lane 2 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : Huh7 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lane 1 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lanes 2-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 105 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST
Exposure time:
Lane 1:3 minutes;
Lane 2:70 seconds;
Lane 3:15 seconds;
Lane 4:3 minutesThe molecular weight observed is consistent with what has been described in the literature (PMID: 19577557).
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![Immunocytochemistry/ Immunofluorescence - Anti-ENPP1/PC1 antibody [EPR22262-22] (ab223268)](https://www.abcam.com/ps/products/223/ab223268/Images/ab223268-319499-anti-enpp1-antibody-epr22262-22-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ENPP1/PC1 antibody [EPR22262-22] (ab223268)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling ENPP1/PC1 (green) with ab223268 at 1/100 dilution, followed by Alexa Fluor® 488 Goat anti-Rabbit secondary antibody (ab150077) at 1/1000 dilution. Confocal image showing membranous staining in HepG2 cells. Tubulin was detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red). The nuclear countertsain was DAPI (Blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Alexa Fluor® 488 Goat anti-Rabbit (ab150077) at 1/200 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-ENPP1/PC1 antibody [EPR22262-22] (ab223268)](https://www.abcam.com/ps/products/223/ab223268/Images/ab223268-319500-anti-enpp1-antibody-epr22262-22-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ENPP1/PC1 antibody [EPR22262-22] (ab223268)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-231 (human breast adenocarcinoma cell line) cells labeling ENPP1/PC1 (green) with ab223268 at 1/100 dilution, followed by Alexa Fluor® 488 Goat anti-Rabbit secondary antibody (ab150077) at 1/1000 dilution. Confocal image showing membranous staining in MDA-MB-231 cells. Tubulin was detected using Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (Red). The nuclear countertsain was DAPI (Blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Alexa Fluor® 488 Goat anti-Rabbit (ab150077) at 1/200 dilution.
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![Immunoprecipitation - Anti-ENPP1/PC1 antibody [EPR22262-22] (ab223268)](https://www.abcam.com/ps/products/223/ab223268/Images/ab223268-319502-anti-enpp1-antibody-epr22262-22-immunoprecipitation.jpg)
Immunoprecipitation - Anti-ENPP1/PC1 antibody [EPR22262-22] (ab223268)ENPP1/PC1 was immunoprecipitated from 0.35 mg of MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate using ab223268 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab223268 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.
Lane 1: MDA-MB-231 whole cell lysate 10μg (input)
Lane 2: ab223268 IP in MDA-MB-231 whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab223268 in MDA-MB-231 whole cell lysate (-).Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds. -
![Flow Cytometry - Anti-ENPP1/PC1 antibody [EPR22262-22] (ab223268)](https://www.abcam.com/ps/products/223/ab223268/Images/ab223268-319501-anti-enpp1-antibody-epr22262-22-flow-cytometry.jpg)
Flow Cytometry - Anti-ENPP1/PC1 antibody [EPR22262-22] (ab223268)Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling ENPP1/PC1 with ab223268 (red) compared with a Rabbit monoclonal IgG isotype control (ab172730) (black)and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP1/PC1 antibody [EPR22262-22] (ab223268)](https://www.abcam.com/ps/products/223/ab223268/Images/ab223268-319498-anti-enpp1-antibody-epr22262-22-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP1/PC1 antibody [EPR22262-22] (ab223268)Immunohistochemical analysis of paraffin-embedded human liver tissue stained for ENPP1/PC1 using ab223268 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous staining on human liver (PMID: 25539584; PMID: 9344668; PMID: 23861746) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP1/PC1 antibody [EPR22262-22] (ab223268)](https://www.abcam.com/ps/products/223/ab223268/Images/ab223268-319497-anti-enpp1-antibody-epr22262-22-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP1/PC1 antibody [EPR22262-22] (ab223268)Immunohistochemical analysis of paraffin-embedded human pancreas tissue stained for ENPP1/PC1 using ab223268 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous staining on human pancreas (PMID: 9344668; PMID: 23861746) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Anti-ENPP1/PC1 antibody [EPR22262-22] (ab223268)](https://www.abcam.com/ps/products/223/ab223268/Images/ab223268-8-benefits-of-recombinant-antibodies.png)
Anti-ENPP1/PC1 antibody [EPR22262-22] (ab223268)
