Anti-DMP1 antibody (ab103203)
Key features and details
- Rabbit polyclonal to DMP1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DMP1 antibody
See all DMP1 primary antibodies -
Description
Rabbit polyclonal to DMP1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow
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Immunogen
Synthetic peptide corresponding to Human DMP1 aa 450 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab103555) -
Positive control
- This antibody gave a positive signal in Human bone marrow tissue lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab103203 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). Target
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Relevance
DMP1 is an extracellular matrix protein and a member of the small integrin binding ligand N-linked glycoprotein family. It is critical for proper mineralization of bone and dentin, and is present in diverse cells of bone and tooth tissues. DMP1 contains a large number of acidic domains, multiple phosphorylation sites, a functional arg-gly-asp cell attachment sequence, and a DNA binding domain. DMP1 may also have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts the unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite. -
Cellular localization
Nucleus. Cytoplasm. Secreted; extracellular space; extracellular matrix. Note=In proliferating preosteoblasts it is nuclear, during early maturation stage is cytoplasmic and in mature osteoblast localizes in the mineralizated matrix. Export from the nucleus of differentiating osteoblast is triggered by the release of calcium from intracellular stores followed by a massive influx of this pool of calcium into the nucleus. -
Database links
- Entrez Gene: 1758 Human
- Entrez Gene: 13406 Mouse
- Omim: 600980 Human
- SwissProt: Q13316 Human
- SwissProt: O55188 Mouse
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Alternative names
- ARHP antibody
- ARHR antibody
- AV020965 antibody
see all
Images
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Western blot - Anti-DMP1 antibody (ab103203)Anti-DMP1 antibody (ab103203) at 1 µg/ml + Bone Marrow (Human) Tissue Lysate - adult normal tissue at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Additional bands at: 50 kDa (possible truncated form)
Exposure time: 4 minutes -
Western blot - Anti-DMP1 antibody (ab103203)Image courtesy of an anonymous Abreview.Anti-DMP1 antibody (ab103203) at 1/200 dilution + whole cell lysate prepared from murine bone marrow stromal osteoblasts at 20000 cells
Secondary
HRP-conjugated goat polyclonal at 1/120000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 30 seconds
Osteoblasts were treated with 50µg/ml L-ascorbic acid, 10 mM betha-glycerophosphate for 7 days.
Datasheets and documents
References (9)
ab103203 has been referenced in 9 publications.
- Cheng Q et al. The Antimicrobial Peptide LL-37 Promotes Migration and Odonto/Osteogenic Differentiation of Stem Cells from the Apical Papilla through the Akt/Wnt/ß-catenin Signaling Pathway. J Endod 46:964-972 (2020). PubMed: 32389381
- Ahn JH et al. The Effect of Mesoporous Bioactive Glass Nanoparticles/Graphene Oxide Composites on the Differentiation and Mineralization of Human Dental Pulp Stem Cells. Nanomaterials (Basel) 10:N/A (2020). PubMed: 32230907
- Dussold C et al. DMP1 prevents osteocyte alterations, FGF23 elevation and left ventricular hypertrophy in mice with chronic kidney disease. Bone Res 7:12 (2019). PubMed: 31044094
- Gulseren G et al. Dentin Phosphoprotein Mimetic Peptide Nanofibers Promote Biomineralization. Macromol Biosci N/A:e1800080 (2018). PubMed: 29745025
- Vijayan V & Gupta S Role of osteocytes in mediating bone mineralization during hyperhomocysteinemia. J Endocrinol 233:243-255 (2017). PubMed: 28507207
- Jiang S et al. Disruption of kif3a results in defective osteoblastic differentiation in dental mesenchymal stem/precursor cells via the Wnt signaling pathway. Mol Med Rep 14:1891-900 (2016). WB ; Human . PubMed: 27432616
- Hase N et al. Products of dentin matrix protein-1 degradation by interleukin-1ß-induced matrix metalloproteinase-3 promote proliferation of odontoblastic cells. Biosci Trends 9:228-36 (2015). PubMed: 26355224
- Lee HK et al. Nuclear factor I-C (NFIC) regulates dentin sialophosphoprotein (DSPP) and E-cadherin via control of Krüppel-like factor 4 (KLF4) during dentinogenesis. J Biol Chem 289:28225-36 (2014). PubMed: 25138274
- Martin A et al. Overexpression of the DMP1 C-terminal fragment stimulates FGF23 and exacerbates the hypophosphatemic rickets phenotype in Hyp mice. Mol Endocrinol 26:1883-95 (2012). IHC-P ; Mouse . PubMed: 22930691
Images
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Western blot - Anti-DMP1 antibody (ab103203)Anti-DMP1 antibody (ab103203) at 1 µg/ml + Bone Marrow (Human) Tissue Lysate - adult normal tissue at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Additional bands at: 50 kDa (possible truncated form)
Exposure time: 4 minutes
-
Western blot - Anti-DMP1 antibody (ab103203) Image courtesy of an anonymous Abreview.Anti-DMP1 antibody (ab103203) at 1/200 dilution + whole cell lysate prepared from murine bone marrow stromal osteoblasts at 20000 cells
Secondary
HRP-conjugated goat polyclonal at 1/120000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 30 seconds
Osteoblasts were treated with 50µg/ml L-ascorbic acid, 10 mM betha-glycerophosphate for 7 days.