Anti-Wnt11 antibody (ab31962)
Key features and details
- Rabbit polyclonal to Wnt11
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Wnt11 antibody
See all Wnt11 primary antibodies -
Description
Rabbit polyclonal to Wnt11 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow
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Immunogen
Synthetic peptide corresponding to Human Wnt11 aa 250-350 conjugated to keyhole limpet haemocyanin.
(Peptide available asab28716) -
Positive control
- This antibody gave a positive signal in MDA-MB-361 whole cell lysate. This antibody gave a positive result in IF in the following formaldehyde fixed cell lines: DU145.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab31962 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ICC/IF Use a concentration of 5 µg/ml. WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 39 kDa). Target
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Function
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. -
Tissue specificity
Expressed in fetal lung, kidney, adult heart, liver, skeletal muscle, and pancreas. -
Sequence similarities
Belongs to the Wnt family. -
Post-translational
modificationsPalmitoylation at Ser-215 is required for efficient binding to frizzled receptors. Palmitoylation is necessary for proper trafficking to cell surface. -
Cellular localization
Secreted, extracellular space, extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 7481 Human
- Entrez Gene: 22411 Mouse
- Entrez Gene: 140584 Rat
- Omim: 603699 Human
- SwissProt: O96014 Human
- SwissProt: P48615 Mouse
- Unigene: 108219 Human
- Unigene: 22182 Mouse
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Alternative names
- HWNT11 antibody
- Protein Wnt-11 antibody
- Wingless-related MMTV integration site 11 antibody
see all
Images
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Western blot - Anti-Wnt11 antibody (ab31962)Anti-Wnt11 antibody (ab31962) at 1 µg/ml + MDA MB 361 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 15 µg
Secondary
Goat polyclonal to rabbit IgG - H&L - Pre Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody (ab31962)ICC/IF image of ab31962 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab31962 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (15)
ab31962 has been referenced in 15 publications.
- Liu Y et al. Chromosome 3q26 Gain Is an Early Event Driving Coordinated Overexpression of the PRKCI, SOX2, and ECT2 Oncogenes in Lung Squamous Cell Carcinoma. Cell Rep 30:771-782.e6 (2020). PubMed: 31968252
- Chen J et al. Nkx2.5 insufficiency leads to atrial electrical remodeling through Wnt signaling in HL-1 cells. Exp Ther Med 18:4631-4636 (2019). PubMed: 31798700
- Spinelli C et al. Molecular subtypes and differentiation programmes of glioma stem cells as determinants of extracellular vesicle profiles and endothelial cell-stimulating activities. J Extracell Vesicles 7:1490144 (2018). PubMed: 30034643
- Cao N et al. MicroRNA-298 represses hepatocellular carcinoma progression by inhibiting CTNND1-mediated Wnt/ß-catenin signaling. Biomed Pharmacother 106:483-490 (2018). PubMed: 29990836
- Zhang X et al. Hyperglycemia Altered the Fate of Cardiac Stem Cells to Adipogenesis through Inhibiting the ß-Catenin/TCF-4 Pathway. Cell Physiol Biochem 49:2254-2263 (2018). PubMed: 30257243
Images
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Western blot - Anti-Wnt11 antibody (ab31962)Anti-Wnt11 antibody (ab31962) at 1 µg/ml + MDA MB 361 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 15 µg
Secondary
Goat polyclonal to rabbit IgG - H&L - Pre Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
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Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody (ab31962)
ICC/IF image of ab31962 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab31962 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
