Anti-Wnt11 antibody (ab96730)
Key features and details
- Rabbit polyclonal to Wnt11
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Wnt11 antibody
See all Wnt11 primary antibodies -
Description
Rabbit polyclonal to Wnt11 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-280 of Human Wnt11 (NP_004617).
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Positive control
- WB: HEK-293T, A431, COLO 205 cell lysate, mouse heart white adipose tissue extract. IHC-P: Lung SCC xenograft and mouse colon tissue. IF: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Wnt11 antibody (ab96730) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 39 kDa10% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt11 antibody (ab96730)
Paraffin-embedded mouse colon tissue stained Wnt11 using ab96730 at 1/500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min
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Immunofluorescence analysis of paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling Wnt11 (green) using ab96730 at 1/200 dilution. Lower image merged with DNA probe.
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Anti-Wnt11 antibody (ab96730) at 1/1000 dilution + Mouse heart tissue extractc at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 39 kDa10% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt11 antibody (ab96730)
Paraffin-embedded lung SCC xenograft satined Wnt11 using ab96730 at 1/100 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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Immunofluorescence analysis of ice-cold MeOH-fixed (5 min) HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling Wnt11 (green) at cytoplasm using ab96730 at 1/500 dilution. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
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Anti-Wnt11 antibody (ab96730) at 1/1000 dilution + Mouse white adipose tissue extract at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 39 kDa10% SDS-PAGE
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Anti-Wnt11 antibody (ab96730) at 1/1000 dilution + COLO 205 (human colon adenocarcinoma cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 39 kDa10% SDS-PAGE