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Anti-Wnt11 antibody (ab96730)

Price and availability

284 784 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-Wnt11 antibody (ab96730)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Wnt11
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Wnt11 antibody
    See all Wnt11 primary antibodies
  • Description

    Rabbit polyclonal to Wnt11
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-280 of Human Wnt11 (NP_004617).

  • Positive control

    • WB: HEK-293T, A431, COLO 205 cell lysate, mouse heart white adipose tissue extract. IHC-P: Lung SCC xenograft and mouse colon tissue. IF: HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Secreted

Images

  • Western blot - Anti-Wnt11 antibody (ab96730)
    Western blot - Anti-Wnt11 antibody (ab96730)
    All lanes : Anti-Wnt11 antibody (ab96730) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 39 kDa



    10% SDS-PAGE

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt11 antibody (ab96730)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt11 antibody (ab96730)

    Paraffin-embedded mouse colon tissue stained Wnt11 using ab96730 at 1/500 dilution. 

    Antigen Retrieval: EDTA based buffer, pH 8.0, 15min

     

  • Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody (ab96730)
    Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody (ab96730)

    Immunofluorescence analysis of paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling Wnt11 (green) using ab96730 at 1/200 dilution. Lower image merged with DNA probe.

  • Western blot - Anti-Wnt11 antibody (ab96730)
    Western blot - Anti-Wnt11 antibody (ab96730)
    Anti-Wnt11 antibody (ab96730) at 1/1000 dilution + Mouse heart tissue extractc at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 39 kDa



    10% SDS-PAGE

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt11 antibody (ab96730)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt11 antibody (ab96730)

    Paraffin-embedded lung SCC xenograft satined Wnt11 using ab96730 at 1/100 dilution.

    Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.

  • Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody (ab96730)
    Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody (ab96730)

    Immunofluorescence analysis of ice-cold MeOH-fixed (5 min) HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling Wnt11 (green) at cytoplasm using ab96730 at 1/500 dilution. Blue: Hoechst 33342 staining. Scale bar = 10 μm.

  • Western blot - Anti-Wnt11 antibody (ab96730)
    Western blot - Anti-Wnt11 antibody (ab96730)
    Anti-Wnt11 antibody (ab96730) at 1/1000 dilution + Mouse white adipose tissue extract at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 39 kDa



    10% SDS-PAGE

  • Western blot - Anti-Wnt11 antibody (ab96730)
    Western blot - Anti-Wnt11 antibody (ab96730)
    Anti-Wnt11 antibody (ab96730) at 1/1000 dilution + COLO 205 (human colon adenocarcinoma cell line) whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 39 kDa



    10% SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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